AIM The aim was to study the polymorphism of exon 2 and exon 3 of growth hormone (GH) gene, to test the polymorphic variants for Hardy-Weinberg equilibrium and to investigate association of these polymorphisms with chest girth and paunch girth in Sirohi and Barbari breeds of goat. MATERIALS AND METHODS A total of 80 kids involving forty each of Sirohi and Barbari breeds of goat were included ...

Several reports have indicated that infection with Non-Tuberculosis Mycobacteria (NTM) is increasing worldwide.Therefore,monitoring species causing micobacterial infection in any region is of great importance. This study was going to detect, differentiate, and identify pathogenic mycobacteria in primary clinical samples. Eighty samples collected from tuberculosis suspected patients in Isfahan/I...

The M-protein genes (emm genes) of 103 separate impetiginous Streptococcus pyogenes isolates were sequenced and the sequence types were compared to the types obtained by Vir typing. Vir typing is based on restriction fragment length polymorphism (RFLP) analysis of a 4- to 7-kb pathogenicity island encoding emm and other virulence genes. By using both HaeIII and HinfI to generate RFLP profiles, ...

We have used enriched chicken histone cDNA to select genomal clones from a chicken library. Because the cDNA probe also contained other sequences, a further screening of positive plagues with negative probes eliminated most non-histone gene clones. One 'positively-selected' genomal clone, lambda CH-01, hybridised with cloned sea-urchin histone genes and also detected histone genes in EcoRI-dige...

 It is one of the most nutritious millet and staple food in different states of India and Pseudomonas sp. MSSRFD41  MSSRFD41 was isolated from finger millet rhizosphere soil samples. 16S rRNA gene sequence shows maximum 98.77% similarity and forms monophyletic clade with closely related species.  It produces 2,4-DAPG, HCN, AHLs, IAA, solubilises phosphate and tolerates 1 M NaCl.  Exhibits p...

The six major hepatitis C virus genotypes were investigated by using samples from 79 seropositive and PCR-positive blood donors from three different regions of South Africa as well as 9 patients with chronic renal failure, 19 with liver disease, and 23 with hemophilia. PCR products of the genome were typed by restriction fragment length polymorphic analysis by RsaI-HaeIII and MvaI-HinfI double ...