Fiber diameter, a quantitative trait, is controlled by minor effect polygenes. Keratin-associated proteins (KRTAPs) are an important part of hair, and their rich polymorphisms facilitate the mining cashmere trait molecular markers. In this study, Jiangnan Tibetan goats were taken as research object; multiplex PCR exome sequencing technology used to identify exon regional KRTAP15-1, KRTAP13.1, K...

The main objective of the current study was to obtain preliminary results on genetic polymorhism of A and C variants at the CSN2 locus in indigenous Romanian goat (Banat White, Carpatina) breeds using a rapid and efficient genotyping method, TaqMan assay (Applied Biosystems, USA). Hair follicle samples were taken from 73 Banat White and 82 Carpatina purebred goats from Arad, Caras-Severin and T...

Precision genetic engineering accelerates the genetic improvement of livestock for agriculture and biomedicine. We have recently reported our success in producing gene-modified goats using the CRISPR/Cas9 system through microinjection of Cas9 mRNA and sgRNAs targeting the MSTN and FGF5 genes in goat embryos. By investigating the influence of gene modification on the phenotypes of Cas9-mediated ...