The biological activity of monoclonal antibodies specific for the hemagglutinin protein of measles virus strain CAM recognizing six epitope groups according to their binding properties to measles virus strain CAM/R401 was investigated in vivo in our rat model of measles encephalitis. When injected intraperitoneally into measles virus-infected suckling rats, some monoclonal antibodies modified t...

background: botulinum neurotoxin (bont) complexes consist of neurotoxin and neurotoxin-associated proteins. hemagglutinin-33 (ha-33) is a member of bont type a (bont/a) complex. considering the protective role of ha-33 in preservation of bont/a in gastrointestinal harsh conditions and also its adjuvant role, recombinant production of this protein is favorable. thus in this study, ha-33 was expr...

objective: influenza virus a (h1n1) is an important subtype of the influenza respiratory viruses, which has important worldwide implications. hemagglutinin (ha), an important viral antigen, is responsible for binding to human cell receptors leading to an onset of the disease process. considering the critical role of viral attachment, this study focuses on the extraction and cloning of ha and it...

Avian influenza viruses of the H5 and H7 hemagglutinin subtypes, and Newcastle disease virus (NDV), are important pathogens in poultry worldwide. Specifically, the highly pathogenic H5N1 avian influenza virus is a particular threat because it has now occurred in more than 40 countries on several continents. Inasmuch as most chickens worldwide are vaccinated with a live NDV vaccine, we embarked ...

Transport from the TGN to the basolateral surface involves a rab/N-ethylmaleimide-sensitive fusion protein (NSF)/soluble NSF attachment protein (SNAP)/SNAP receptor (SNARE) mechanism. Apical transport instead is thought to be mediated by detergent-insoluble sphingolipid-cholesterol rafts. By reducing the cholesterol level of living cells by 60-70% with lovastatin and methyl-beta-cyclodextrin, w...

A hemagglutinin was purified from the coelomic fluid of the sea urchin Anthocidaris crassispina by ion-exchange chromatography on DEAE-cellulose and affinity adsorption to glutaraldehyde-fixed ghosts of human erythrocytes, followed by elution with 10 mM EDTA. On sodium dodecyl sulfate-polyacrylamide gel electrophoresis, it showed a single protein band with a molecular weight of 13 000 and 26 00...