The childhood bone marrow failure disorders are a heterogeneous group of diseases of which the commonest ones are acquired severe aplastic anemia (SAA) and myelodysplastic syndromes. SAA is characterized by the presence of pancytopenia with a hypocellular marrow in the absence of malignant infiltration or fibrosis. Myelodysplastic syndromes are characterized by ineffective hematopoiesis, dyplas...

We developed here a new mathematical modelling approach which reconciliates native and stress hematopoiesis, focused on hematopoietic stem progenitor compartments. first proposed an erythropoiesis model leaning minimum of 6 cell-amplification compartments, able to reproduce erythropoiesis. A phenylhydrazine-induced hemolytic was next applied, and in vivo data were used estimate parameters throu...

Blood development proceeds through several waves of hematopoietic progenitors with unclear lineage relationships, which convolute the understanding process. Thinking precursors as “blood germ layer” can integrate these into a unified that originates in yolk sac, earliest site blood development. Hematopoietic differentiation pluripotent stem cells (PSCs) reflects to certain extent complexities s...

Human T-cell lymphotropic virus type 1 (HTLV-1) is the etiologic agent of adult T-cell leukemia, an aggressive CD4 malignancy. Although HTLV-2 is highly homologous to HTLV-1, infection with HTLV-2 has not been associated with lymphoproliferative disorders. Lentivirus-mediated transduction of CD34 cells with HTLV-1 Tax (Tax1) induced G0/G1 cell cycle arrest and resulted in the concomitant suppre...

We evaluated the methylation status of the X-linked gene phosphoglycerate kinase (PGK1) and the DXS 255 locus detected by probe M27 beta to study clonality in acquired aplastic anemia (AA). A total of 30 females were suitable for clonal analysis of peripheral blood polymorphonuclear cells (PMN) and mononuclear cells using a polymerase chain reaction-based procedure in 24 patients and Southern b...

Abstract Isocitrate dehydrogenase (IDH) mutations are common genetic alterations in myeloid disorders, including acute leukemia (AML) and myelodysplastic syndrome (MDS). Epigenetic changes, abnormal histone DNA methylation, have been implicated the pathogenic build-up of hematopoietic progenitors, but it is still unclear whether how IDH themselves affect hematopoiesis. Here, we show that IDH1-m...

The development of mature blood cell from hematopoietic stem cells is regulated by transcription factors that coordinate the expression of lineage-specific genes. GATA transcription factors are zinc finger DNA-binding proteins that play crucial roles in various biological processes, including hematopoiesis. Among GATA family proteins, GATA-1, GATA-2, and GATA-3 are essential for hematopoiesis. ...

Myelodysplastic syndromes are a heterogeneous group of clonal hematopoietic stem cell disorders characterized by ineffective hematopoiesis, peripheral cytopenias, and a variable propensity for leukemic transformation. In recent years there has been an explosion of information on the molecular genetic changes underlying these disorders. This information has substantial prognostic implications, a...

The chemokine stromal cell–derived factor-1 (SDF-1) is constitutively expressed by bone marrow stromal cells and plays key roles in hematopoiesis. Fibroblast growth factor 2 (FGF2), a member of the FGF family that plays important roles in developmental morphogenic processes, is abnormally elevated in the bone marrow from patients with clonal myeloid disorders and other disorders where normal he...

PURPOSE OF REVIEW Vasculogenesis and hematopoiesis are essential for development. Recently, the ETS domain transcription factor Etv2 has been identified as an essential regulator of vasculogenesis and hematopoiesis. Here, we review the recent studies that have established the critical role of Etv2 in the specification of mesoderm to blood and endothelial cells. RECENT FINDINGS Loss and gain-o...