Overexpression of the c-Myc transcription factor in liver induces glucose uptake and utilization. Here we examined the effects of c- myc overexpression on the expression of hepatocyte-specific transcription factor genes which regulate the expression of genes controlling hepatic metabolism. At 4 months after streptozotocin (STZ) treatment, most diabetic control mice were highly hyperglycaemic an...

Hepatic hypothermia can safely prolong the duration of hepatic inflow occlusion during complex liver resectional surgeries. The mechanism(s) by which hypothermia protects against this form of liver ischemia-reperfusion injury are not completely understood. In this study, we sought to determine whether hypothermia protects against ischemia-reperfusion injury by altering the hepatic inflammatory ...

AIM To investigate the differentiation potential of human umbilical mesenchymal stem cells (HuMSCs) and the key factors that facilitate hepatic differentiation. METHODS HuMSCs were induced to become hepatocyte-like cells according to a previously published protocol. The differentiation status of the hepatocyte-like cells was examined by observing the morphological changes under an inverted mi...

The hyperlipidemia accompanying infection has been attributed to production of tumor necrosis factor. This cytokine inhibits adipose tissue lipoprotein lipase, which could decrease clearance of lipoproteins. Infections also increase hepatic lipogenesis. We now have demonstrated that tumor necrosis factor-alpha stimulates lipid synthesis in vivo. 2 h after administration of tumor necrosis factor...

L-2-oxothiazolidine-4-carboxylic acid (OTC) is a cysteine prodrug that maintains glutathione in tissues. Here, its effect on alcohol-induced liver injury in an enteral alcohol feeding model was investigated. Male Wistar rats were given control high-fat or ethanol containing diets enterally for 4 weeks. Treated rats received 500 mg/kg/d of dietary OTC. Ethanol delivery, weight gain, and the cycl...

background: there is no convenient cheap pragmatic experimental model for nonalcoholic fatty liver disease (nafld)/nonalcoholic steatohepatitis (nash). we aimed to create a pragmatic model of nafld/nash. methods: sprague-dawley rats were fed a high-fat, high sugar homemade diet ad libitum for seven weeks. the high-fat, high sugar diet included 59% of energy derived from fat, 30% from carbohydra...

Temporally distinct groups of cytokine expression was observed by reverse transcription-polymerase chain reaction assay, in situ hybridization, and immunohistochemistry in the livers of Listeria monocytogenes-infected mice. One group consisted of interferon-gamma (IFN-gamma), tumor necrosis factor alpha (TNF-alpha), and interleukin-10 (IL-10), for which mRNAs were induced within 1 day after cha...