DNA coding for the core antigen from hepatitis B Virus (HBcAg) was amplified, cloned and propagated in E. coli. The core protein was expressed in E. coli and the product was readily detected by Western blot. This protein can be used as a diagnostic material in serum screening tests. To increase the level of expression of this antigen in bacteria, two plasmids were constructed in which the gene ...

Background: Genomic studies of hepatitis B virus (HBV) diversity are becoming increasingly significant to understand how HBV mutations interact with a wide spectrum clinical and pathological disorders. Objective: This study focused on identifying genotypes determining the status pre-core (PC) core promoter (CP) mutants. Methods: Nested polymerase chain reaction was used identify viral 100 patie...

background and aims: lamivudine is amongst the antiviral for drug chronic hepatitis b treatment. during therapy with lamivudine, variants may emerge with ymdd mutation in the reverse transcriptase (rt) region of polymerase gene. this mutation might have a role in drug resistant for hbv. materials and methods: hbv dna extraction from serum sample of 88 patients, were subjected to nested pcr for ...

BACKGROUND: The acute Hepatitis B virus (HBV) infection usually ceases before six months, but chronic that lasts for more than months might develop into liver cirrhosis and hepatocellular carcinoma (HCC). Viral particle load, HBV genotypes association to the x (HBx) gene mutations are probable factors related HCC occurrence. mutation which leads HBx T118N was found as second most common in Indo...

this report describes a 66-year-old male who had a long history of hepatitis b virus (hbv) infection. he was found hepatocellular carcinoma (hcc) 5 months after lamivudine resistance mutation and then received a successful hepatectomy. three years later, hepatitis b envelope antigen seroconversion was achieved and nucleoside analogs were discontinued. after the withdrawn of antiviral treatment,...

Objective(s) The aim of this study was to characterize the hepatitis B virus surface protein genotypes and sequence variations among hepatitis B virus surface antigen (HBsAg) positive chronic patients in Hormozgan province, south of Iran. Materials and Methods A total of 8 patients enrolled in this study. The surface gene was amplified and directly sequenced. Genotypes and nucleotide/amino a...

background although certain hbv mutations are known to affect the expression of hepatitis e antigen, their association with hbv viral level or clinical outcomes is less clear. objectives we evaluated associations between different mutations in the basal core promoter (bcp) and pre-core (pc) regions of hbv genome and subsequent changes in hbv viral dna level over seven years in a population of u...

conclusions this study indicates that 2.9% of hemodialysis patients had obi, which is considered as a major public health problem worldwide. moreover, we observed three mutations in s region, including t127p, p153l, and f170s, which caused obi. this study is first to report a mutation analysis of hbv in hemodialysis patients in southwestern iran. these results indicate that current screening te...

 ABSTRACT Background: Hepatitis B virus (HBV) infection in patients who lack detectable hepatitis B surface antigen (HBsAg) is called occult hepatitis B infection. Such infections have been frequently identified in patients with chronic hepatitis Cliver disease, but their prevalence is not known. Methods: 207 patients with chronic hepatitis C who were HCV -RNA and antiHCV positive were stu...

background hepatitis b virus (hbv) infection is a major public health problem that affects billions of people worldwide. the lack of information on hbv prevalence among the general population is an obstacle to formulate effective policies to reduce the burden of viral hepatitis. objectives this population based serological survey was conducted in kermanshah province to determine the local preva...