BACKGROUND Gradual development of a useful vaccine can be the main point in the control and eradication of Hepatitis C virus (HCV) infection. Hepatitis C Virus envelope glycoproteins are considered as the main HCV vaccine candidate. OBJECTIVES In this study, the Pichia pastoris expression system was used to express a recombinant HCV CoreE1E2 protein, which consists of Core (269 nt-841nt) E1 (...

conclusions hcv gpe2 is an ideal target for hcv vaccine. prediction of epitope immunogenicity and characterization on the basis of peptide sequences will be significantly helpful for development of a heterologous vaccine against hcv variants. results many b-cell and t-cell epitopes predicted in gpe2 were found conserved among hcv 3a genotypes whereas few were conserved in other genotypes antici...

background the hepatitis c virus (hcv) infection has been identified as a leading cause of progressive liver diseases worldwide. despite new treatment strategies, pegylated interferon alfa-2a (peg-ifnα-2a), in combination with ribavirin (rbv), still represents the gold standard of therapy for hepatitis c in developing countries. results in this study, a significant association was observed betw...

Hepatitis C virus (HCV) is continuing to spread worldwide, adding three million new infections each year. Currently approved therapies are highly effective; however, access to them is limited due to the high cost of treatment. Therefore, a cost effective vaccine and alternative antivirals remain essential. HCV envelope glycoproteins, E1 and E2, heterodimerize on the virion surface and are the m...

WEST, JOHN ALLEN. Investigation of the Role of the E2 Endodomain in Sindbis Virus Assembly. (Under the direction of Dr. Dennis T. Brown) Sindbis virus (SV) is the prototype member of the alphavirus genus belonging to the family Togaviridae. SV is a mosquito-borne virus that can be transmitted to both humans and other animals via mosquito bloodmeal. Structurally, SV is composed of three proteins...

The hepatitis C virus (HCV) is a small enveloped RNA virus belonging to the family flaviviridae and genus hepacivirus. The HCV RNA genome is 9,600 nucleotides in length and encodes a single polyprotein that is post-translationally cleaved into 10 polypeptides including t3 structural (C, E1, and E2) and multiple nonstructural proteins ([NS] NS2 to NS5). The NS proteins include enzymes necessary ...

The transmembrane (TM) domains of hepatitis C virus (HCV) envelope glycoproteins E1 and E2 have been shown to play multiple roles during the biogenesis of the E1E2 heterodimer. By using alanine scanning insertion mutagenesis within the TM domains of HCV envelope glycoproteins, we have previously shown that the central regions of these domains as well as the N-terminal part of the TM domain of E...

conclusions the current study found a relatively high frequency of positivity gbv-c-exposure among the patients receiving hemodialysis in the area understudy. nosocomial transmission seems to be the main route of gbv-c infection in the area. objectives the current study aimed to evaluate gbv-c infection among hemodialysis patients. patients and methods totally, 149 patients receiving hemodialys...

Hepatitis C virus (HCV) is a major cause of chronic hepatitis worldwide. Studies of the early steps of HCV infection have been hampered by the lack of convenient in vitro or in vivo models. Although several cell-surface molecules that mediate the binding of HCV envelope proteins to target cells have been identified, mechanisms of viral entry into human hepatocytes are still poorly understood. V...