Un patient âgé de 23 ans, pécheur, issu de parents non consanguins qui présentait une polyarthrite déformante associant un flessum irréductible des coudes et des genoux avec limitation des amplitudes articulaires. Ces manifestations articulaires étaient associées à des nodules localisés en regard des articulations atteintes (A,B) et au niveau des pavillons des oreilles. La biologie révèle une V...

Interspecific human-mouse and Chinese hamster-mouse hybrids were isolated from polyethylene glycol fused cells by a new half-selection technique employing a structurally modified polyene macrolide antibiotic, amphotericin B methyl ester (AME), and HAT media. Unfused parental cells were killed as a result of innate sensitivity to AME or their genetic deficiency, absence of thymidine kinase (TK-)...

Purine enzyme activities are usually assayed by radiochemical procedures and often TLC is part of the separation method. In screening patients with rheumatic diseases, these procedures have shown disadvantages like a relatively large coefficient of variation (C.V.) and time-instability. We describe a non-radiochemical reversed-phase HPLC micro-method with UV detection for measurement of activit...

The mutagenicity of emission samples from three oil-fired and four coal-fired boilers have been compared by using the Salmonella/microsome assay. Very little or no mutagenic activity was observed in samples from five of these boilers. The sample from one oil-fired boiler showed mutagenic activity of about 500 revertants/MJ, and the sample from a coal-fired fluidized bed combustor had an activit...

Studies of human purine metabolism began some 200 years ago with Scheele's identification of uric acid as a constituent of a renal calculus' and Wollaston's demonstration of urate in a tophus from his own ear.2 Just as these were among the earliest experiments in the new science of biochemistry, so Garrod's (1854)3 thread test must rank as the first assay in what we now call the discipline of c...

Abbreviations: small cell lung cancer (SCLC); non-small cell lung cancer (NSCLC); intratumoral injection of Ad-p53 (INGN 201); murine double minute-2 (MDM2); cisplatin (CDDP); active metabolite of irinotecan (CPT-11); O(6)-methylguanine-DNA methyltransferase (MGMT); retinoblastoma (RB); fragile histidine triad (FHIT); tumor necrosis factor-related apoptosis-inducing ligand (TRAIL); triplex form...

Hybrids between A9 (HGPRT-) and B82 (TK-), mouse heteroploid fibroblast lines, were obtained through continuous cultivation and clonal selection; such hybrids showed marked segregation and by conventional stains displayed chromosome numbers and distribution similar to that of either parental type. Detailed analyses by Giemsa (G)- and centromeric-banding of these parental lines, and of 4 of the ...

Plasmodium falciparum is dangerous malarial species and most burdensome form of human malaria, affecting 200–300 million individuals per year worldwide, also because of its high rate of resistance outbreaks; there is a constant need for the discovery of novel antimalarials and drug targets. The three-dimensional structures of PFHGPRT, HSHGPRT and TCHGPRT were used for comparative docking study,...