background: recently, we have shown that peroxisomal protein expression was induced upon retinoic acid treatment in mouse embryonic stem cells during the process of neurogenesis. thus, characterization of the respective promoter could elucidate the molecular aspects of transcriptional regulation of this gene. methods: using the conventional software programs for promoter prediction, a putative ...

Abstract Lack of appropriate tools for visualizing cell membrane molecules at the nanoscale in a non‐invasive and label‐free fashion limits our understanding many vital cellular processes. Here, we use tip‐enhanced Raman spectroscopy (TERS) to visualize molecular distribution pancreatic cancer (BxPC‐3) membranes ambient conditions without labelling, with spatial resolution down ca. 2.5 nm. TERS...

Symfoil-4P is a de novo protein exhibiting the threefold symmetrical β-trefoil fold designed based on the human acidic fibroblast growth factor. First three asparagine-glycine sequences of Symfoil-4P are replaced with glutamine-glycine (Symfoil-QG) or serine-glycine (Symfoil-SG) sequences protecting from deamidation, and His-Symfoil-II was prepared by introducing a protease digestion site into ...

In Escherichia coli as many as nine different genes coding for proteins with significant homology to peptidyl-prolyl cis/trans-isomerases (PPIases) have been found. However, for three of them, the histidine-rich SlyD, the homologous gene product of ORF149, and parvulin-like SurA, it was not known whether these proteins really possess PPIase activity. To gain access to the full set of PPIases in...

The substitution of 1-methyl-l-histidine for the histidine heme ligands in a de novo designed four-alpha-helix bundle scaffold results in conversion of a six-coordinate cytochrome maquette into a self-assembled five-coordinate mono-(1-methyl-histidine)-ligated heme as an initial maquette for the dioxygen carrier protein myoglobin. UV-vis, magnetic circular dichroism, and resonance Raman spectro...