The presence of acetylcholinesterase in the tumour cells of neuroblastoma has been shown by enzyme histochemistry. For comparison, some other tumours likely to be found in children and commonly presenting histologically as small cell tumours have also been studied. Acetylcholinesterase activity was seen in rhabdomyosarcoma, but, compared with neuroblastoma, the activity was focal and sparse. On...

Cryostat sectioning is a popular but labor-intensive method for preparing histological brain sections. We have developed a modification of the commercially available CryoJane tape collection method that significantly improves the ease of collection and the final quality of the tissue sections. The key modification involves an array of UVLEDs to achieve uniform polymerization of the glass slide ...

Since the introduction by Jones and Zimmerman (I 959, I 96 I) of a reliable histological scheme for the separate recognition of each of the three major categories of corneal stromal dystrophy, the way has been open for precise characterization of the nature of the lesions in each of these conditions. This has been largely accomplished for the macular and lattice dystrophies, the former having b...

SASAI, Y. Lactic Dehydrogenase Activity in Human Epidermis: Semiquantita tive Histochemical Study. Tohoku J. exp. Med ., 1972, 108 (4), 327-336 The graphical method of Lineweaver-Burk to the histoehemical system was applied to obtain quantitative information on the lactic dehydrogenase activity in human epidermis. In vitro experiment showed that the incubation time necessary for yielding a cert...

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Potassium concentrations in excess of 30 mM increase the rate of beating of lateral cilia on the gill of Mytilus edulis. Cilioexcitation produced by low frequency (5 beats/s) electrical stimulation was potentiated with potassium but blocked with bromolysergic acid (a serotonergic inhibitor). Cilioinhibition produced by high frequency (50 beats/s) stimulation was decreased with potassium and phe...

A developmental progression of histochemical fiber typing is demonstrated in normal rat soleus muscle. By utilizing the acid lability of ATPase, the type II fibres are subdivided into type IIA, IIB and IIC. No IIB fibers are present in the newborn or adult rat soleus. In the 1-day-old animal 90% of the fibers can be differentiated into type I and II with all of the type II's demonstrating IIC c...

Oral Cleansing, Spiders, Gnathocoxal Gland, Histochemistry, Micromorphological Investigations Histochemical and micromorphological investigations of the gnathocoxal gland in spiders brought out its uniquely adaptive cleansing function. The prey particulates, caught in the oral mi­ crostructures, are bound with mucopolysaccharides or glyco-muco-proteins from the glandular se­ cretions in Araneus...

Low glomerular (nephron) endowment has been associated with an increased risk of cardiovascular and renal disease in adulthood. Nephron endowment in humans is determined by 36 wk of gestation, while in rats and mice nephrogenesis ends several days after birth. Specific genes and environmental perturbations have been shown to regulate nephron endowment. Until now, design-based method for estimat...