BACKGROUND Sting challenge with a live insect remains the best test for proving the efficacy of immunotherapy in Hymenoptera allergy. OBJECTIVE We studied the impact of tolerated sting challenge on quality of life. PATIENTS AND METHODS In this prospective study, data were collected via self-report questionnaires completed by consenting patients with Hymenoptera venom allergy on venom immuno...

AIM To evaluate markers of mast cell and basophil activation in children undergoing the initial phase of honeybee venom immunotherapy (VIT). PATIENTS & METHODS Five children (four boys and one girl) aged 9.5-18 years with severe systemic bee sting reactions and confirmed IgE-mediated allergy were enrolled. Plasma and urine concentrations of 9α,11β-PGF2 and serum tryptase levels were measured ...

The aim of this study was to assess the response to a honeybee venom by analyzing serum levels of 34 free amino acids. Another goal of this study was to apply complex analytic-bioinformatic-clinical strategy based on up-to-date achievements of mass spectrometry in metabolomic profiling. The amino acid profiles were determined using hybrid triple quadrupole/linear ion trap mass spectrometer coup...

“Look back into the nature and then you will understand everything better.” This quote by Albert Einstein clearly point towards the paradigm shift from costlier antibiotic therapies to the old tradition remedies with minimum side effects like apitherapy. Apitherapy is the medicinal use of products made by honeybees. Therapies involving the honeybee have existed for thousands of years and some m...

For the past 10 years, beekeeping has increased due to a growing awareness of the disappearance of bees since Colony Collapse Disorder. Most of the disappearance of honey bees can be attributed to the use of pesticides. Apitherapy is the science and art of maintaining health with the use of products from the honeybee hive: honey, bee pollen, propolis, royal jelly, and bee venom. We have been be...

The aim of the study was to the development of the method for the separation, identification and determination quantitatively of the major constituents of honeybee (Apis mellifera L.) venom by high performance liquid chromatography (HLPC) and determination of the chemical composition of the domestic product by running routine tests. In the study to develop an HPLC method to assay the major veno...