A miniaturized extraction/preconcentration method based on an aqueous biphasic system (μ-ABS) was developed with reagents commonly used as food additives: cholinium chloride (ChCl) main extraction phase, K2HPO4 salting-out agent, and water the component (being sample for analyses). With aim of obtaining high enrichment factors, miniaturization, adequate analytical performance, a point in region...

In this study, simultaneous quantification of allantoin, uric acid, xanthine, and hypoxanthine in cow milk by solid phase extraction (SPE) high performance liquid chromatography-diode array detection (HPLC-DAD) method was perform. Five different SPE cartridges were tested order to evaluate the isolation purine derivatives (PD) from milk. Chromatography carried out on ODS-2 Hypersil column 0.05 ...

The extraction of red beetroot (Beta vulgaris) peel betalains and phenolics was compared with two extraction methods and solvents. The content of total phenolics in the extracts was determined according to a modification of the Folin-Ciocalteu method and expressed as gallic acid equivalents (GAE). The profiles of extracts were analysed by high-performance liquid chromatography (HPLC). The compo...

In this study, the carotenoid profile of the echinoderm Marthasterias glacialis L. was established using HPLC-DAD-APCI-MS/MS equipped with a C(30) column. This approach rendered the identification of 20 compounds, eight of them reported for the first time in this marine organism. Differentiation of carotenoid isomers was also achieved.

BACKGROUND In the present study the antioxidant potential of a methanol extract of Phyllanthus emblica leaves (PELE) was determined by in vitro methods as well as by an in vivo animal model, along with HPLC-DAD screening for phyto-constituents. METHODS The in vitro antioxidant potential of PELE was assessed by scavenging of DPPH, nitric oxide and anti-lipid peroxidation assays. For in vivo ev...

Dan-Deng-Tong-Nao capsules (DDTNCs), a traditional Chinese medicinal preparation consisting of Radix Salvia miltiorrhiza, Radix Pueraria lobata, Erigeron breviscapus, Rhizoma chuanxiong has been used for the treatment of apoplexy and the syndrome of apoplexy involving both collaterals and meridians caused by obstruction of collaterals by blood stasis in China. In this paper, a HPLC-DAD-MS/MS me...

INTRODUCTION: Primaquine (PQ) diphosphate is an 8-aminoquinoline antimalarial drug with unique therapeutic properties. It is the only drug that prevents relapses of Plasmodium vivax or Plasmodium ovale infections. In this study, a fast, sensitive, cost-effective, and robust method for the extraction and high-performance liquid chromatography with diode array ultraviolet detection (HPLC-DAD-UV )...