Background and objectives:Drimia maritima (L.) Stearn also known as squill is a medicinally important plant that has been used for various ailments such as dropsy, respiratory disorders, jaundice and epilepsy from ancient times. Proscillaridin A is identified as one of the most effective compounds in the plant with remarkable pharmacological features including efficacy...


 Purpose: To develop a reversed phase high performance liquid chromatography (HPLC) method for the determination of dehydroepiandrosterone (DHEA) in dietary supplements.
 Methods: A reversed-phase was developed DHEA supplements. An isocratic system consisting methanol and water (70:30 v/v) run at flow rate 1 mL/min on C18 HPLC column to achieve separation. The validated with regard l...

Abstract A sensitive and accurate high performance liquid chromatography method utilizing ultraviolet/visible light detection (HPLC-UV) for the quantification of Ribociclib in Spiked Human Plasma by HPLC-UV was developed validated. (RCB) internal standard (IS), Trifluridine, were first extracted from plasma samples a simple Protein Precipitation extraction using Acetonitrile. concentration RCB ...

Nicotinamide adenine dinucleotide (NAD(+)) is a coenzyme for hydride transfer reactions and a substrate for sirtuins and other NAD(+)-consuming enzymes. The abundance of NAD (+), NAD(+) biosynthetic intermediates, and related nucleotides reflects the metabolic state of cells and tissues. High performance liquid chromatography (HPLC) followed by ultraviolet-visible (UV-Vis) spectroscopic analysi...

This paper described a fast, simple, and small scale method of sample preparation followed by High-Performance Liquid Chromatography (HPLC) coupled Photo-Diode Array (PDA) detector for quantification of canthaxanthin in chicken liver and fat, and hen’s egg yolk. The HPLC-PDA was performed on a C18 column with an isocratic mobile phase. Analyte was extracted from the sample using a handheld ultr...

The purpose of this study was to evaluate the influence of tobacco smoke on the pharmacokinetics of citalopram (CIT) and desmethylcitalopram (DCIT) and its enantiomers on an animal model. High performance liquid chromatography (HPLC) with a diode array detector (DAD) was used for the identification and quantification of the studied compounds. The HPLC quantification of racemic mixtures of CIT w...

BACKGROUND Simple, reproducible assays are needed for the quantification of sphingolipids, ceramide (Cer), and sphingoid bases. We developed an HPLC method for simultaneous quantification of total plasma concentrations of Cer, glucosylceramide (GlcCer), and ceramide trihexoside (CTH). METHODS After addition of sphinganine as internal calibrator, we extracted lipids from 50 microL plasma. We d...

The development of facile and rapid quantification of biologically active biomolecules such as isotretitoin in therapeutic drugs contained in many generic formulations is necessary for determining their efficiency and their quality to improve the human health care. Isotretritoin finds its applications in the maintenance of epithelial tissues. Different processes to date such as normal phase HPL...