Objective: Boswellic acids (BAs) are active constituents of plant Boswellia serrata. In the present study boswellic acids were extracted from Boswellia serrata and dry methanolic extracts were quantified by HPLC and HPTLC. Method: A gradient HPLC method was used for the quantification of the boswellic acids; 11-keto-β-boswellic acid (KBA) and 3acetyl-11-keto-β-boswellic acid (AKBA). A direct HP...

Hundred Fusarium culmorum strains, isolated from freshly harvested maize grain samples from Southern parts of India, were incubated in czapek-dox medium and analyzed for trichothecene (DON/NIV) production. The mPCR assay was standardized targeting trichothecene metabolic pathway genes viz., Tri6, Tri7, Tri13 for detection of trichothecene (DON/NIV) chemotypes and rDNA gene for specific detectio...

To estimate phenols and flavonoids, enzymic antioxidants in the leaves of Boerhaavia erecta (LBE). The sample was extracted with 0.1M phosphate buffer for the analysis of phenols, flavonoids and antioxidants. The total phenolic content and total flavonoids in LBE were measured using Folin-Ciocalteau and aluminium chloride colorimetric methods respectively. The activity of Superoxide Dismutase (...

BACKGROUND Nowadays, plants have been considered as powerful agents for treatment of disorders due to their traditional use. Plants have a special role in the treatment of various diseases in Ayurveda. Liver disorders with their devastating outcomes have been discussed in Ayurveda as well. OBJECTIVES In the present study, polyherbal products (L52 and L38) were retrieved from Ayurveda and its ...

Corchorus depressus (L.) Stocks syn. C. depressus (L.) C. Chr. (Tiliaceae), commonly known as Baphuli, traditionally used in pain, fever, sexual dysfunction and to treat gonorrhea. In the present investigation, various pharmacognostical standards for C. depressus have been established. Collection of plant material was carried out and its morphological, physico-chemical and phytochemical studies...

Mucuna pruriens Linn. is an important medicinal plant used for treatment of Parkinson's disease and many others in ancient Indian medical system. L-DOPA extracted from seeds of Mucuna is a constituent of more than 200 indigenous drug formulations and is more effective as drug than the synthetic counterpart. A densitometric high performance thin-layer chromatographic (HPTLC) method was developed...

Alpinetin is a flavonoidal constituent of seeds of Amomum subulatum Roxb., recently reported to possess vasorelaxant and antiHIV activities. Simple, accurate and precise HPLC and HPTLC methods were developed for the analysis of alpinetin in A. subulatum seed extracts and extraction technique was optimized to get maximum yield using conventional, ultrasonic and matrix solid phase dispersion extr...

Simple, accurate and precise UV spectrophotometric and chromatographic methods have been developed and validated for the determination of tiapride in its pharmaceutical formulation. The two analytical methods UV spectroscopy and HPTLC were developed and validated according to the experimental conditions of the study. The UV spectroscopic analysis was performed using methanol as solvent and dete...

A simple, rapid, and reliable HPTLC method has been established for simultaneous determination of Nebivolol and S-Amlodipine in pharmaceutical dosage forms. Identification and quantification were performed on silica gel 60 F254 HPTLC plates, prewashed with methanol, with chloroform:toluene:methanol:glacialacetic acid, (5:2:3:0.1, v/v/v/v), as mobile phase. This system furnished compact bands fo...

Dashmulakwatha, a preparation of Dashmula official in the Ayurvedic Formulary, was estimated for its phytosterol content by HPTLC method. The preparation was found to contain 7.165% W/W of phytosterols with respect to β-sitosterol as standard.