Background: The functional longevity of ovarian transplant at present is one of the most challenging questions regarding ovary transplantation. So, we used hyaluronic acid (HA) hydrogel scaffold with combination of VEGF and bFGF for restoration of cyclical ovarian function after ovarian tissue transplantation in a rat model. Materials and Methods: A total of 25 Wistar female rats weighing betwe...

A series of 16 benign human ovarian neoplasms of either stromal or epithelial origin, with one exception, showed transfer RNA methylase specific activity and tRNA-methylating capacity comparable to that of normal ovarian tissue and not to malignant ovarian tumor tissue. The exception was an arrhenoblastoma, a rare masculinizing type of tumor which, although classified as benign, is known to hav...

oocyte, embryo and ovarian tissue cryopreservation are being increasingly proposed for fertility preservation among cancer patients undergoing therapy to enable them to have babies after the cancer is cured. embryo cryopreservation is not appropriate for single girls without any sperm partner and also because oocyte retrieval is an extended procedure, it is impossible in cases requiring immedia...

OBJECTIVE miR-613 may suppress ovarian cancer development and progression. We wished to assess the potential prognostic value of miR-613 expression analysis in ovarian cancer. PATIENTS AND METHODS A total of 236 pairs of human ovarian cancer and matched normal tissue specimens were collected. Resected tissue specimens were obtained during the operation and were snap-frozen in liquid nitrogen ...

BACKGROUND Controlled-rate freezing of ovarian cortical tissue for preservation of fertility among young women facing chemo- or radio-therapy is a widely accepted procedure. To improve the method for cryopreservation of ovarian tissue, particularly the stroma, we carried out a systematic comparison of vitrification versus slow programmed freezing. METHODS Ovarian tissue from 20 women, donated...

This study examined the relationship between the expression of p16, a tumor suppressor gene, and lymph node metastasis, as well as patient prognosis, in cases with ovarian cancer. SKOV-3, an ovarian cancer cell line, and IOSE80, a normal human ovarian cell line, were selected for testing. Western blot analysis was used to detect the p16 expression in ovarian cell culture samples. In the study, ...

background: in vitro maturation (ivm) of immature oocytes collected from ovary has been proposed for fertility preservation. in addition, quality of oocytes post ivm is one of the factors determining its developmental competence. by using the non-invasive polscope system, both meiotic spindle (ms) and zona pellucida (zp) can be assessed in living oocytes. objective: the aim was to investigate t...

Sir, In the challenging paper by Wang et al. (2008), the authors have reported about an effective method of vitrification of human ovarian tissue with direct contact of cells in liquid nitrogen, named needle immersed vitrification. Childbirth after cryopreservation of ovarian tissue is now a reality (Donnez et al., 2004; Demeestere et al., 2007; Meirow et al., 2007; Andersen et al., 2008) and t...

Ovarian freezing and transplantation has garnered increasing interest as a potential way of preserving fertility in cancer patients as well as for women who just wish to delay childbearing. This chapter spells out our techniques of ovarian cortex vitrification and results for frozen compared to fresh ovarian cortex transplantation (in one single series from one center for the sake of consistenc...