The first equine pregnancy using sperm cryopreserved in glycerol was reported in 1957,1 and glycerol is still considered the optimal cryoprotectant for equine sperm.2–4 However, this opinion is largely based on data from other species, and research that evaluates additional cryoprotective agents (CPA’s) specifically for equine sperm is lacking. Other compounds such as ethylene glycol, dimethyl ...

In this study, certain enzymes in ram semen involved in reactive oxygen species elimination and their changes during the cryopreservation process were characterized in order to investigate the hypothesis that the antioxidant defense system is involved in the maintenance of frozen sperm quality. Glutathione reductase (GR), glutathione peroxidase (GPx), and superoxide dismutase (SOD) activities w...

The first methods for fertility preservation (FP) were sperm freezing followed many years later by ovarian tissue freezing and oocyte vitrification. FP before cancer treatment is necessary because gametes are often destroyed at the same time as the cancer therapy kills malignant cells. It has been regularly offered to male cancer patients in the form of sperm cryopreservation. In female patient...

In the present study, the fertilizing potential of semen recovered from slaughtered bulls epididymis was evaluated after cryopreservation, by conventional techniques and flow cytometry methods. The cauda epididymal was dissected and sperm were recovered and evaluated for volume, sperm concentration, and membrane and acrosome integrity using a flow cytometer. Sperm fertility potential was tested...

The effect of seminal plasma, as well as some seminal plasma fractions, on the heterogeneity and viability of frozen-thawed ram spermatozoa was studied. Fresh and frozen sperm samples were simultaneously assessed by a routine semen-quality assay and centrifugal countercurrent distribution (CCCD) in an aqueous two-phase system analysis. All samples used for this study were obtained from Salz ram...

The fertilizing ability of stallion semen was analyzed using fresh and frozen samples, obtained before (June-July) or during (October-November) the breeding season. Thirty ejaculates obtained from 4 stallions were used. The analysis comprises routine seminogram; ATP concentration (Comhaire et al., 1983); subjective and objective motility and sperm velocity (Makler, 1980). Freezing was done foll...

Objective To investigate effects of cryopreservation on changes of the ultrastructure of human testicular sperm and evaluate the efficacy of cryopreserving testicular tissue as a source of sperm for assisted reproduction. Methods Testicular biopsy tissues were obtained from infertile patients (n=12) with obstructive azoospermia and cryopreserved. Testicular sperm motility was observed after in ...

important aspect of sperm function such as motility and capacitation appear to be mediated at least partially though hyaloronic acid (ha). present study investigated effects of different doses of ha on sperm motility and vitality in human. sperm was obtained from 20 male from ivf clinic in imam khomeini hospital. sperm motility and vitality in human semen was analyzed according to who criteria ...

Twentieth-century researcher Ernest John Christopher Polge studied the reproductive processes of livestock and determined a method to successfully freeze, thaw, and utilize viable [4] sperm [5] cells to produce offspring in animals. In 1949, Polge identified glycerol as a cryoprotectant, or a medium that enables cells to freeze without damaging their cellular components or functions. Several ye...