background: alzheimer’s disease (ad) is a progressive neurodegenerative disease and nowadays the role of endothelial cell (ec) injury has been proposed in pathological process in ad. peroxisome proliferator‑activated receptor‑γ (ppar‑γ) agonist has anti‑inflammatory properties through activation in glial cells and improves vascular function and prevent atherosclerotic disease progression. the a...

background: angiogenesis, the formation of new blood vessels from preexisting ones, is one of the most important physiological and pathological processes occur in the body. in the pathological conditions such as tumor growth, psoriasis, corneal neovascularization and rheumatoid arthritis, angiogenesis is crucially required for the devolopment of the disease. zataria multiflora as a member of la...

Resveratrol is a polyphenolic compound which found in many nutrients including grapes, peanuts, raspberries, and apples. Anti-proliferative, anti-angiogenic apoptotic effects of resveratrol have been shown on various cancer cells. Doxorubicin considered as one the most effective anticancer agents reveals its antitumor activity by induction apoptosis inhibition angiogenesis. Our study reports fo...

AIM To investigate the killing efficiency of a recombinant plasmid containing a thymidine kinase (TK) domain insert driven by the vascular endothelial growth factor receptor 2 (VEGFR2) promoter (KDR) on vascular endothelial cells. METHODS The KDR-TK fragment was extracted from pBluescript II KDR-TK plasmid by enzymatic digestion with XhoI and SalI. The enhanced green fluorescence protein (EGF...

Short interfering RNA (siRNA) is a powerful technique that can suppress gene expression in a variety of cells including mammalian cells. Endothelial progenitor cells (EPCs) are bone marrow—derived haematopoietic progenitor cells that have been implicated in vasculogenesis. We demonstrated for the first time that gene silencing of endothelial E-selectin using siRNA transfection in human umbilica...

Purpose: To investigate the protective effects of ligustrazine on oxidative stress-induced atherosclerosis. Methods: The indicators related to oxidative stress were determined using commercially available assay kits. MTT assay was used to assess the survival rate of human umbilical vein endothelial cells (HUVECs). HUVECs apoptosis was analyzed using fluorescence staining and flow cytometry. mRN...

Differences in the secretion of some vascular regulators, endothelin-1 (ET-1), prostacylin (PGI2), thromboxane A2 (TXA2) and nitric oxide (NO) associated with in vitro aging, were investigated in cultured endothelial cells isolated from normal human umbilical veins (HUVECs). HUVECs from different population doubling levels (PDLs) were cultured in a medium MCDB-104 supplemented with FBS and ECGF...

introduction: pulsed dye laser (pdl) is an important treatment for superficial infantile hemangioma, but few studies report on its cellular mechanism. the aim of this study was to evaluate alterations of serum vascular endothelial growth factor (vegf) level in infantile hemangioma (ih) patients after laser treatment and effects of pdl irradiation on human umbilical vein endothelial cells (huvec...

The use of biodegradable beta-tricalcium phosphate (β-TCP) scaffolds holds great promise for bone tissue engineering. However, the effects of β-TCP on bone and endothelial cells are not fully understood. This study aimed to investigate cell proliferation and differentiation of mono- or co-cultured human-bone-marrow-derived mesenchymal stem cells (hBMSCs) and human-umbilical-vein endothelial cel...

Dysfunction of endothelial cells in severe malaria may result from excessive activation of tumor necrosis factor (TNF)-α which leads to an increase in production of reactive oxygen species (ROS) and decrease of antioxidant level of endothelial cells. To investigate the effect of N-acetylcysteine (NAC) on hydrogen peroxide (H2O2), malondialdehyde (MDA) and glutathione (GSH) levels produced by en...