OBJECTIVE Human atherosclerotic lesions have been shown to contain lipid droplets and vesicles resembling those of in vitro enzymatically modified LDL. However, little is known about the hydrolytic enzymes in the arterial intima that induce fusion of LDL particles and so produce lipid droplets or that induce foam cell formation. METHODS AND RESULTS Human coronary atherosclerotic lesions obtai...

We studied the possibility that the antagonistic saprobe fungi Coriolopsis rigida, Trametes versicolor, Fusarium lateritium, Penicillium chrysogenum and Verticillium dahliae-2379 may control V. dahliae disease through the inhibition of the activity of hydrolytic enzymes produced by the pathogen. These saprobe fungi were able to decrease the growth in vitro of V. dahliae. The exudates produced b...

A study was conducted at Universiti Putra Malaysia, Malaysia, glasshouse to determine the inoculation effect of Sb42 (locally isolated diazotroph) with different doses of nitrogen fertiliser (0, 30 and 60kg N ha) on hydrolytic enzyme production and the growth of rice variety MR219. Using 16S rRNA partial gene sequencing, Sb42 was identified as Bacillus sp. Plants were harvested at 45 days after...

The biological interest of oligosaccharides is growing very rapidly, and necessitates the development of efficient synthesis reactions. The stereoand regio-selectivity of enzyme catalysis is a key advantage in this field, as a complementary tool to the chemical approach. Two types of enzymes can be applied to the obtention of oligosaccharides: Hydrolytic enzymes, which can catalyze either rever...

The detection of small amounts (nanomoles) of inorganic phosphate has a great interest in biochemistry. In particular, phosphate detection is useful to evaluate the rate of hydrolysis of phosphatases, that are enzymes able to remove phosphate from their substrate by hydrolytic cleavage. The hydrolysis rate is correlated to enzyme activity, an extremely important functional parameter. Among phos...

As an alternative to screening in the directed evolution of enantioselective enzymes, a selection system has been implemented for a lipase-catalyzed hydrolytic kinetic resolution of a chiral ester.

The hydrolase content of vacuoles isolated from protoplasts of suspension-cultured tobacco cells, of tulip petals, and of pineapple leaves, and the sedimentation behavior of tobacco tonoplasts were studied. Three precautions were found to be important for the analysis of vacuolar hydrolases and of the tonoplast. (a) Purification of protoplasts in a Ficoll gradient was necessary to remove cell d...