Recent studies have shown that the hydroxyproline and hydroxylysine found in the collagen of vertebrates are synthesized by the hydroxylation of proline and lysine, which have been incorporated into a large polypeptide precursor of collagen called protocollagen. Because the collagen isolated from the cuticle of Ascaris lumbricoides contains less hydroxyproline and hydroxylysine than any other k...

Urinary hydroxyproline excretion was measured in 130 chain saw operators aged 28-59 and in 31 normal control subjects aged 26-59 with no occupational exposure to vibration. The results measured were expressed as a ratio of hydroxyproline to creatinine. (1) No significant correlation between hydroxyproline/creatinine ratio and age was observed among normal subjects. (2) In chain saw operators gr...

BUCCINO, R. A., E. HARRIS, J. F. SPANN, JR., AND E. H. SONNENBLICK. Response of myocardial connective tissue to development of experimental hypertrophy.. Am. J. Physiol. 2 16(2) : 425-428. 1969.-Myocardial collagen concentration was determined by measuring hydroxyproline in right and left ventricles of normal. cats and those with right ventricular hypertrophy produced by chronic constriction of...

The effects of electric fields of different intensities and directions on the collagen synthesis of guinea pigs was studied. The effects of vertical and horizontal electric fields of 1.9 kV/m and 0.9 kV/m, generated with voltages of 300 V and 150 V DC, respectively, on collagen synthesis, was evaluated by assessing the amount of hydroxyproline in the liver tissue, and by histological examinatio...

Light and electron microscopy of resorbing connective tissues have shown that the collagen fibers and fibrils disappear. It is usually presumed that resorbed collagen is degraded proteolytically to hydroxyproline-containing peptides and to free amino acids, including hydroxyproline. We wish to present evidence that collagen can be resorbed and then reutilized without such breakdown. Urinary exc...

Collagen hydroxyproline is an important posttranslational modification activity because of its close relationship with various diseases and signaling activities. However, there is no study to date for constructing models for predicting collagen hydroxyproline sites. Support vector machines with two kernel functions (the identity kernel function and the bio-kernel function) have been used for co...

Zorab, P. A., Clark, S., Harrison, A., and Seel, J. R. (1970). Archives of Disease in Childhood, 45, 763. Hydroxyproline excretion and height velocity in adolescent boys. The height velocity of 122 boys of 11 to 17 years is compared with their urinary total hydroxyproline excretion and a close relation is shown. The importance of this is discussed in relation to undersized children and to those...

The growth of the j T6 mouse fibroblast and synthesis of macromolecules has been studied over the last 6 days of the cultures' 14-day life span. The effect of ascorbic acid was also tested. During this period, the cells synthesized collagen, as judged by the appearance of nondialysable hydroxyproline, which was identified by chemical assay and by radioactive incorporation studies. A high propor...

Passive stiffness and hydroxyproline content of myocardium hypertrophied by pressure-loading were determined in kittens 2, 8-16, and 24-52 wk after pulmonary artery banding, which initially elevated right ventricular systolic pressure by 10-15 mm Hg. Right ventricular mass increased by approximately 75%, three-quarters of which occurred during the first 2 wk after banding. Passive stiffness was...

Injury to the capillary endothelium and to alveolar epithelial cells of the lung may result in damage to the underlying collagen of the extracellular matrix. To examine this possibility, whole body irradiation, bleomycin injections, and exposure to hyperoxia were used to induce various types of lung damage in mice. The morphology of the lung and the cellular and protein content of bronchoalveol...