This study was conducted to determine the effects of different culture times on root growth and accumulation secondary metabolites in adventitious roots from Hyoscyamus niger petioles. For this purpose, transferred media containing 1 mM methyl jasmonate (MeJA) were harvested at 11 times, starting day 30. After harvest, fresh dry weights index as parameters growth, well amounts tropane alkaloids...

Background: Black henbane with the scientific name of Hyoscyamus niger which has a wide geographic distribution, is considered as one the most important species for tropane alkaloids extraction. Objective: In this research, morphological and tropane alkaloids variations were assessed within different population of H. niger in their natural habitats. Methods: Totally, 56 individuals were colle...

Heavy metal pollution is a worldwide problem with serious environmental consequences. The objective of the present experiment was to investigate whether arginine as nitric oxide precursor and or polyamines substrate can decrease the destructive effects of oxidative stress induced by nickel contamination in Hyoscyamus niger plant. In this study the effects of arginine pretreatment on alkaloid co...

The distribution of poly(A)-containing RNA [poly(A)+RNA] in pollen grains of Hyoscyamus niger during normal gametophytic development and embryogenic development induced by culture of anther segments was followed by in situ hybridization with [3H]-polyuridylic acid as a probe. No binding of the isotope occurred in pollen grains during the uninucleate phase of their development. Although [3H]poly...