Reverse transcription-PCR (RT-PCR) has traditionally required time-consuming RNA extraction and purification. This report demonstrates that one can completely avoid the RNA extraction step in RT-PCR by basing the comparison of samples on cell number rather than micrograms of total RNA. A new method for lysing cells while preserving RNA is described. RT-PCR is carried out (i) by rapidly freezing...

403 Assay result variability during determination of mismatch repair deficiency status using immunohistochemistry: A transatlantic comparative study. Hutchins G., Gray G., Quirke P. Abstract #491 Use of a multigene prognostic assay for selection of adjuvant chemotherapy in patients with stage II colon cancer: Impact on qualityadjusted life expectancy and costs. Meropol N.J., Lyman G.H., Chien R...

Bu çalışmada, Antalya ili Finike ilçesi marul üretim alanlarında Marul mozayik virüsü (Lettuce mosaic virüs: LMV)’nün belirlenmesi amacıyla yürütülmüştür. Virüsün tanılama çalışmalarında DAS-ELISA (Double Antibody Sandwich Enzyme Linked Immunosorbent Assay) ve IC-RT-PCR (Immunocapture Ters Transkripsiyon Polimeraz Zincir Reaksiyonu) yöntemleri kullanılmıştır. amaçla araştırma alanından 92 örneğ...

outbreaks causing mass mortalities have been observed in the golden grey mullet (liza auratus) population in southern coastal area of the caspian sea since 2002. the clinical signs include neurological abnormalities, such as erratic swimming behavior, spiral swimming, belly-up at rest and over inflation of the swim bladder. three hundred and twelve samples of moribund fish with sizes that range...