True ileal lysine digestibilities were determined using the homoarginine (HA) method in casein-, barley-, canola meal-, and barley-canola meal-based diets fed to growing pigs. Four Yorkshire barrows (25 to 49 kg BW) equipped with ileal T-cannulas were fed one of the diets according to a 4 x 4 Latin square design. All diets, except for the barley diet, were formulated to be similar in DE:CP rati...

Two studies were conducted to assess the effect of dietary protein reduction on N utilization, N excretion, and AA digestibility in growing pigs. The objective was to determine whether pigs fed diets with a reduced CP concentration could maintain the same N retention as pigs fed an adequate diet. The second objective was to test whether reducing dietary CP concentration decreases AA digestibili...

The regression method was used in a 14-d broiler chicken study to determine the true ileal digestibility of amino acid (AA) and protein utilization in canola meal (CM, 388 g of CP/kg) without or with added phytase. Experimental treatments consisted of 2 factors, phytase at 2 levels (0 or 1,500 phytase units/kg) and CM at 3 levels (125, 250, or 375 g/kg). Birds received a standard starter diet f...

Musilova A., Lichovnikova M., Kupcikova L., Anderle V. (2017): Effect of suboptimal levels of non-phytate phosphorus and exogenous phytase on precaecal digestibility of phosphorus and calcium in laying hens. Czech J. Anim. Sci., 62, 473–481. The precaecal digestibility of phosphorus and calcium in laying hens was evaluated at two dietary levels of non-phytate phosphorus (NPP; 1.58 and 2.03 g/kg...

It is important to be able to characterise foods and feedstuffs according to their available amino acid contents. This involves being able to determine amino acids chemically and the conduct of bioassays to determine amino acid digestibility and availability. The chemical analysis of amino acids is not straightforward and meticulousness is required to achieve consistent results. In particular a...

Ileal digestibility is widely used in diet formulation for pigs, and is normally determined using T-piece or re-entrant cannulas. Ileal cannulation allows multiple sampling from a single animal and causes little disturbance to the digestive tract. Leakage around the cannula is prevalent, however, and the studies are consequently highly labour intensive. An alternative is the intact ileal dissec...

Ileal digesta were collected via a post-valve-T-caecum cannula from piglets ( lo-15 kg) fed diets based on raw pea or on pea protein isolate (Finale or Frijaune variety). From the determined amino acids (AA) content of feed and digesta, AA apparent digestibility coefficients and proportions of proteins (endogenous, bacterial, dietary) theoretically present in digesta were calculated. Four essen...

Digestibility of diets based on corn and soybean meal or soybeans treated by roasting or extrusion, with or without an enzyme supplementation, was measured by "true" (Sibbald) methods, by analysis of excreta, and by analysis of ileal digesta. Only analysis of ileal digesta was able to consistently measure differences between soybean and enzyme treatments in the digestibility of CP, starch, fat,...

Growth and N utilization responses of pigs to Aspergillus niger phytase supplementation of low-protein or high-phytin diets were examined in 2 experiments. In the first experiment, 6 diets arranged in a 3 x 2 factorial with Aspergillus niger phytase at 0, 600, or 1,200 units/kg and dietary protein at 160 or 200 g/kg; and 12 pigs (growth) or 6 pigs (N utilization) per diet were used to test the ...

Ileally cannulated pigs were used to assess the effects of four dietary levels of microbial phytase (Natuphos) on the apparent and true digestibility of Ca, P, CP, and AA in dehulled soybean meal. Fourteen pigs (25 kg initial BW) were surgically fitted with T-cannulas at the terminal ileum and assigned to diets in a replicated 7 x 7 Latin square design. Following a 14-d recovery, four diets con...