Growing Bacillus subtilis cells were immobilized in k-carrageenan gel beads. Growth and sporulation of entrapped cells were studied by two different methods: cell enumeration and transmission electron microscopy. Immobilized growing cells had a shorter generation time than free cells did, whereas sporulation timing was unchanged. When steady state was reached, cell density was very high in gel ...

Human interleukin-10 (IL-10) inhibits T-cell proliferation and cytokine production in the presence of monocytes. In this study, we have investigated whether IL-10 can directly inhibit T cells. Highly purified peripheral blood T cells containing less than 0.1% CD14+ cells and unresponsive to phytohemagglutinin (PHA), were growth-inhibited by IL-10 when stimulated with immobilized OKT3 monoclonal...

Glycosylation of bisphenol A, which is an endocrine disrupting chemical, was investigated using immobilized marine microalga and plant cells from the viewpoint of bioremediation of bisphenol A. Immobilized marine microalga of Amphidinium crassum glucosylated bisphenol A to the corresponding glucoside. On the other hand, bisphenol A was glycosylated to its glucoside, diglycoside, gentiobioside, ...

The aim of the present investigation was to characterize a xylanaseproducing Fusarium solani isolate and to optimize cultural conditions for xylanase enzyme production from free and immobilized cells. Screening of Fusarium solani isolate was based on the diameter of the clear zone formation in oat spelt xylan agar plates. Fusarium solani isolate F7 was selected and optimized for xylanase enzyme...

In the continuous systems, such as continuous beer fermentation, immobilized cells are kept inside the bioreactor for long periods of time. Thus an important factor in the design and performance of the immobilized yeast reactor is immobilized cell viability and physiology. Both the decreasing specific glucose consumption rate (q(im)) and intracellular redox potential of the cells immobilized to...

In this study, modified granular activated carbon (GAC) and immobilized cells were used to improve the biological efficiency of high-ammonia-nitrogen wastewater treatment using microorganisms. The results showed that sodium-hydroxide-modified (NaOH-GAC) greatly increased numbers ammonia-oxidizing bacteria Alcaligenes sp. TD-94 Paracoccus TD-10. Using NaOH-GAC number by 63.27% over GAC. Compared...

aacel9a [β-1,4-endoglucanase, (e.c:3.2.1.4)], was immobilized onto glutaraldehyde activated chitosan macrosphere by covalent attachment. the properties of the immobilized aacel9a were investigated by determining the optimum ph and optimum temperature for activity, thermal stability, and kinetic parameters. the immobilization process shifted the enzyme’s optimum temperature from 65 °c for the fr...

The effect of serum on cell growth and monoclonal antibody (MAb) productivity was studied in a repeated fedbatch mode using both free-suspended and immobilized S3H5/gamma2bA2 hybridoma cells. In the suspension culture, serum influenced the cell growth rate but not the specific MAb productivity. The average specific growth rate of the suspension culture in medium containing 10% serum was approxi...