OBJECTIVE To expatiate dynamic changes in hamsters infected with scrapie strain 263K, to observe the presence and aggravation of various forms of PrP and PrP(Sc) during incubation period, and to probe primarily the relationship between the onset of clinic manifestations and the presence of different PrP(Sc) forms. METHODS Hamster-adapted scrapie strain 263K was intracerebrally inoculated into...

1. This study presents an easy protocol to measure the amount of immunoglobulins from the blood serum of different bird species in the wild ( Ficedula hypoleuca Pallas, Parus caeruleus L., Lanius meridionalis Temminkck, Lanius collurio L., Athene noctua Scopoli and Falco tinnunculus L.) by direct enzyme-linked immunosorbent assay, ELISA using commercial antichicken antibodies. 2. Additionally, ...

Canine leishmaniasis is an endemic disease in the Mediterranean area caused by the protozoan Leishmania infantum, which usually produces renal failure. Sodium dodecyl sulphate polyacrylamide gel electrophoresis and Western blot using antibodies to IgG and IgA from dogs were carried out in the urine of 22 dogs with leishmaniasis diagnosed by ELISA and confirmed by PCR, and 20 healthy dogs. The r...

Soy sauce is a staple fermented food in Korea, however, there are relatively limited studies on soy Korea compared to that Japan. In this study, the immuno-stimulating activities polysaccharides isolated from and Crude was isolated, which were named as Korean-style (KSS-0) Japanese-style (JSS-0). Then, difference cytokine production peritoneal macrophages, KSS-0 showed higher than JSS-0. The pu...

The relationship between the sperm-coating antigens of rabbit seminal plasma and the phenomenon of decapacitation was studied using agar-gel diffusion, immuno-electrophoresis, chromatography on Sephadex G-200, and polyacrylamide vertical gel electrophoresis. Interpretation of data obtained with these techniques led to the conclusion that a sperm-coating antigen of seminal plasma origin possesse...

A method is described for estimating the size of large circular DNAs found within complex chromosomal DNA preparations. DNAs are treated with low levels of gamma-irradiation, sufficient to introduce a single double-stranded break per circle, and the resulting linear DNA is sized by pulsed-field electrophoresis and blot hybridization. The method is fast, reproducible, and very conveniently appli...

The major outer membrane protein patterns of 45 Escherichia coli strains of human origin were compared with that of E. coli K12 by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Preparations of the former strains contained between two and five major bands in the molecular weight range between 30 000 and 42 000. The patterns were very heterogeneous with respect to the numbers and el...