BACKGROUND HER2 testing in gastric cancer differs from testing in breast cancer because of inherent differences in tumor biology; gastric cancer more frequently shows HER2 heterogeneity and incomplete membrane staining. The aim of the present study was to evaluate the frequency and accuracy of detection of HER2 expression by application of standard criteria in Japanese patients with gastric can...

Fluorescent in-situ hybridization (FISH) and immunohistochemistry (IHC) constitute a pair of complimentary techniques for detecting gene amplification and overexpression, respectively. The advantages of IHC include relatively cheap materials and high sample durability, while FISH is the more accurate and reproducible method. Evaluation of FISH and IHC images is still largely performed manually,...

Objective: Melanocytic neoplasia shows different pathways that have been implicated in tumorigenesis. One of the key genes involved BRAFV600E gene, can be detected through DNA-based or protein-based tests including immunohistochemistry (IHC). To check its reproducibility, this study was conducted to evaluate IHC by observers a range melanocytic neoplasms. Methods: The present performed immunohi...

Abstract Immunohistochemistry (IHC) is the use of antibodies as probes to determine localization proteins in a cell or tissue sample. In microscopy, technique has been since 1940s by pathologists and research scientists. At first glance it appears straightforward procedure. practice, however, an IHC protocol many steps where problems can occur resulting either false positive negative data. be u...

Patients with ALK gene rearrangements often manifest dramatic responses to crizotinib, an ALK inhibitor. Accurate identification of patients with ALK-positive non-small cell lung cancer (NSCLC) is essential for the clinical application of ALK-targeted therapy. However, assessing EML4-ALK rearrangement in NSCLC remains challenging in routine pathology practice. The aim of this study was to compa...

Feline infectious peritonitis (FIP) is a systemic disease of cats caused by highly pathogenic variant feline coronavirus, or FCoV. Two distinct genotypes FCoV exist (also referred to as serotypes): Type 1 viruses constitute the vast majority FIP cases, while type 2 are responsible for remaining infections. Immunohistochemistry (IHC) currently serves gold standard diagnosis FIP; however, IHC lim...

Ewing sarcoma arises in both bones (most common) and soft tissues it commonly affects young adults. The tumor is composed of small round cells showing positivity for CD99 FLI1 on immunohistochemistry (IHC). We describe ganglion cell differentiation post-chemotherapy which a rare phenomenon. A 13-year-old girl presented with chest wall mass. On biopsy correlating IHC, the diagnosis was rendered ...

BACKGROUND Data on the accuracy of conventional histomorphologic diagnosis are limited, especially in mucormycosis. We therefore investigated the accuracy of histomorphologic diagnosis of mucormycosis and aspergillosis, using immunohistochemistry (IHC) tests for mucormycosis and aspergillosis. METHODS Patients enrolled met the modified criteria for proven and probable mucormycosis (during a 2...

PTEN deletion is an established prognostic biomarker in prostate cancer. We compared PTEN immunohistochemistry (IHC) and PTEN fluorescence in situ hybridization (FISH) in the largest existing radical prostatectomy cohort with clinical follow-up data. There was high concordance between IHC and FISH: 93% (3098/3330) of tumors with intact PTEN IHC showed absence of PTEN gene deletion and 66% (720/...