Changes in concentrations of 17beta-estradiol (E2) and androgenic hormones were measured in turkey embryos incubated in long-term, shell-less culture (ex ovo) and in ovo. Blood samples were obtained from both sets of embryos on Days 14, 16, 18, 20, and 22 and from embryos incubated in ovo on Days 24, 26, and 28. Ex ovo and in ovo embryos showed no differences in either hormone within sexes, wit...

This study aims to determine the effect of in-ovo injection L-arginine into local chicken eggs on embryo development and chick performance chicken. A total 160 were incubated using semi-automatic incubator with temperature 37-38C relative humidity 55-65%. The injected solution has a concentration 0.5% (m/v). In treatment was divided 4 groups, first without (control), second 0.2 mL (m/v)/egg, th...

Effects of the in ovo injection of commercial diluent supplemented with dextrin or with dextrin in combination with various other carbohydrates on the somatic characteristics and liver nutrient profiles of Ross × Ross 708 broiler embryos and chicks were investigated. Results include information concerning the gluconeogenic energy status of the liver before and after hatch. Eggs containing live ...

Utilization of tightly linked ovo-A vs. ovo-B germline promoters results in the expression of OVO-A and OVO-B, C(2)H(2) transcription factors with different N -termini, and different effects on target gene transcription and on female germline development. We show that two sex-determination signals, the X chromosome number within the germ cells and a female soma, differentially regulate ovo-B an...

the 440 fertile eggs, in a completely randomized design were divided into 11 treatments with four replicatesand 10 eggs per each replicate. treatments including: (t1; control; without injection), (t2; injected with 0.3ml saline 9.0%; sham control), (t3, t4 and t5) 25, 75, 125 ppm fe-nano, (t6, t7 and t8) 50, 100, 150 ppmfe-nano-alimet chelate, (t9, t10 and t11) 50, 100, 150 ppm fe-aliment chela...

Genetic analyses of Drosophila oogenesis have revealed the central role of ovo, a gene required for differentiation of the female germline. A number of recessive ovo mutations also affect the shavenbaby (svb) function required for late embryo patterning, suggesting a tight structural link between ovo and svb. By using various genomic probes for in situ hybridization to wild type and mutant embr...

Two experiments assessed the efficacy of in ovo administration of insulin-like growth factor-I (IGF-I) to enhance skeletal muscle development and improve feed efficiency of broilers. Hatching eggs were divided into three groups: uninjected control, vehicle-injected control, and recombinant human (rh) IGF-I (100 ng per embryo). Eggs in Experiment 1 were injected on Day 1, 4, or one of Day 7 thro...

We have previously demonstrated that short oligodeoxynucleotides containing unmethylated CpG motifs (CpG ODNs) exert a positive effect on weight loss and oocyst shedding associated with Eimeria infection when injected in vivo. The present work investigated the effects of in ovo vaccination with CpG ODNs and an Eimeria recombinant microneme protein (MIC2), alone or in combination, on susceptibil...

This study was conducted to investigate the effect of in ovo feeding (IOF) of folic acid on the folate metabolism, immune function and the involved epigenetic modification of broilers. A total of 400 (Cobb) hatching eggs were randomly divided into four groups (0, 50, 100 and 150 µg injection of folic acid at embryonic age 11 d), and chicks hatched from each treatment were randomly divided into ...