Objective(s): The aim of this study was to prepare fraction and determine the biological activities of the polyphenol-enriched fraction of Berberis integerrima Bunge fruits. Materials and Methods: In this assay fraction was extracted by column chromatography, using Amberlite column as the stationary phase. Phenol and flavonoids in the extract and fraction were analyzed by high performance liqui...

Diverse studies suggest that non-steroidal anti-inflammatory drugs (NSAIDs) induce antinociception through inhibition of cyclooxygenases. In this study was evaluated the effect of NSAIDs to induce antinociception either alone or in combination in the formalin orofacial test of rodent. Male mice were injected intraperitoneally with dexibuprofen, dexktoprofen, diclofenac meloxicam, metamizol, and...

Introduction: Garlic (Allium sativum) is widely used as a flavor-enhancing dietary ingredient and exhibits wide spectrum of pharmacological effects. This study aimed to investigate the therapeutic effects aqueous garlic extract explore bioactivity against 15-lipoxygenase (15-LOX) mediated inflammopathies. Methods: In this study, antioxidant (DPPH free radical scavenging assay reducing power ass...

A dot immunobinding assay ( DIA) was used for a quantitative and qualitative assay of rubella antibody. Purified antigen and conjugated anti-human immunoglobulin (RAHlg) were prepared. Nitrocellulose paper dotted with the antigen was added to serially diluted sera or blood samples. The reacting antibodies were visualized by a peroxidase system. Development of a colored insoluble substrate ...

glutathione s-transferase (gst) represents the major class of detoxification enzymes from helminth parasites such as fasciola hepatica and f. gigantica and it is a candidate for chemotherapeutic and vaccine design. therefore, gst enzyme of fasciola spp. could be a target for evaluation of drugs such as triclabendazole (c14h9cl3n2os). for this purpose, gst enzymes were purified from fasciola spp...

Several assays were compared for their ability to detect inhibition of gap junctional communication in human embryocarcinoma cells exposed to the pesticide dieldrin. Included in this evaluation was a recently developed assay based on the transfer of the fluorescent dye derived metabolically from 5(and 6)-carboxy-2,7-dichlorofluorescein diacetate. This assay was compared to assays for fluorescen...

G9a has been reported to highly express in bladder transitional cell carcinoma (TCC) and G9a inhibition significantly attenuates cell proliferation, but the underlying mechanism is not fully understood. The present study aimed at examining the potential role of autophagy in the anti-proliferation effect of G9a inhibition on TCC T24 and UMUC-3 cell lines in vitro. We found that both pharmaceutic...

To explore the antioxidant potential of hen egg white lysozyme N-acetylmuramide Glycan hydrolase in full fat Mozzarella cheese, an experiment was conducted where control sample brined without addition lysozyme; while four samples were along with 0.02%;w/v (T1), 0.04%;w/v (T2), 0.06%;w/v (T3) and 0.08%;w/v (T4) lysozyme. The cheese measured through several methods, including 2, 2-azino-bis (3-et...

We compared concentrations of antithrombin III (AT-III) in plasma, as determined by an immunological method and by a functional thrombin inhibition method, in the presence of heparin in 160 blood samples from Type I diabetics. Although the correlation was highly significant (P less than 0.001) between the results obtained by the two methods, our data demonstrated that results by the thrombin in...