Many technologies, such as cell line screening and host cell engineering, culture media optimization and bioprocess optimization, have been proposed to increase monoclonal antibody (mAb) production in Chinese Hamster Ovary (CHO) cells. Unlike the existing biochemical approaches, we investigated stimulation using low-intensity pulsed ultrasound (LIPUS) as a purely physical approach, offering enh...

With the rapidly growing demand for monoclonal antibody (mAb)-based products, new technologies are urgently needed to increase mAb production while reducing manufacturing costs. To solve this problem, we report our research findings of using low-intensity pulsed ultrasound (LIPUS) to enhance mAb production. LIPUS with frequency of 1.5 MHz and pulse repetition frequency of 1 kHz, as well as duty...

We explored the therapeutic effects of low-intensity pulsed ultrasound (LIPUS) on a rat model ovarian damage induced by cyclophosphamide. A total 44 female rats with premature insufficiency cyclophosphamide were randomly divided into two groups (an group and control group); 22 normal without also included as third group. The was treated LIPUS, while other received same treatment but any power o...

BACKGROUND Low-intensity pulsed ultrasound (LIPUS) is reported to have the effects of rapid appearance and early maturation of ossification in animal models. METHOD We examined the influence of LIPUS on bone formation in C57BL/6J mouse muscle induced by gene transfer of BMP-4 expression plasmid. Electroporation was employed to transfer plasmid DNA. First, an in vitro study was carried out to ...

INTRODUCTION Chronic left ventricular (LV) pressure overload causes relative ischemia with resultant LV dysfunction. We have recently demonstrated that low-intensity pulsed ultrasound (LIPUS) improves myocardial ischemia in a pig model of chronic myocardial ischemia through enhanced myocardial angiogenesis. In the present study, we thus examined whether LIPUS also ameliorates contractile dysfun...

BACKGROUND There are no evidence on the effects of low-intensity pulsed ultrasound (LIPUS) on surgically managed fresh fractures. We therefore performed a multicenter retrospective cohort study to investigate the effects of LIPUS on surgically managed fresh fractures. METHODS This study included patients surgically treated for diaphyseal fractures of the femur or tibia between August 2009 and...

BACKGROUND Orthognathic surgery can cause discomforts such as pain, inflammation, and edema. One of the challenges is bone regeneration at surgery area. The aim of this study is to evaluate the effect of low-intensity pulsed ultrasound (LIPUS) therapy on bone regeneration and pain relief after surgery. MATERIALS AND METHODS Following mandibular surgery of nine patients, LIPUS treatment was ap...

INTRODUCTION: The medical application of low-intensity pulsed ultrasound (LIPUS) is an established therapy for fracture repair. Despite its pronounced effects during fracture repair, the underlying mechanisms of action of LIPUS remain unclear. Cell-to-cell communication is critical for bone development and remodeling. In the bone marrow, nonhematopoietic-bone marrow stromal cells (BMSC) provide...

Low-intensity pulsed ultrasound (LIPUS) as an adjuvant therapy in in vitro and in vivo bone engineering has proven to be extremely useful. The present study aimed at investigating the effect of 30 mW/cm2 LIPUS stimulation on commercially available human mesenchymal stem cells (hMSCs) cultured in basal or osteogenic medium at different experimental time points (7, 14, 21 days). The hypothesis wa...

Poor patient compliance or nonadherence with prescribed treatments can have a significant unfavorable impact on medical costs and clinical outcomes. In the current study, voice-of-the-customer research was conducted to aid in the development of a next-generation low-intensity pulsed ultrasound (LIPUS) bone healing product. An opportunity to improve patient compliance reporting was identified, r...