The basidiomycetous yeast Trichosporon faecale is considered to be a non-pathogenic fungus; however, the microorganism has been isolated from clinical specimens in several countries. Trichosporon faecale is classified as type I, II, or III depending on the sequence of the intergenic spacer (IGS) region in its rRNA gene. In this study, 28 T. faecale strains obtained from Japanese subjects and en...

The portion of the internal transcribed spacer 1 found on 20S pre-rRNA accumulates in Saccharomyces cerevisiae lacking 5'----3' exoribonuclease 1, showing that an endonucleolytic cleavage at the 3' terminus of 18S rRNA is involved in the 20S pre-rRNA to 18S mature rRNA conversion. Smaller fragments of the spacer sequence are also found. The exoribonuclease may be involved as a cytoplasmic RNase...

Internal Transcribed Spacer (ITS) is one of the most used barcoding regions for molecular phylogenetics and orchids. Our aim in this study to test reliability ITS on closely related Neotinea spp., including tridentata, ustulata subsp. aestivalis, by comparing it accD-psaI intergenic spacer plastid DNA. Both were amplified specific primer sets sequenced. Phylogenetic trees regenerated using Maxi...

The utility of microsatellites (SSRs) in reconstructing phylogenies is largely confined to studies below the genus level, due to the potential of homoplasy resulting from allele size range constraints and poor SSR transferability among divergent taxa. The eucalypt genus Corymbia has been shown to be monophyletic using morphological characters, however, analyses of intergenic spacer sequences ha...

Investigations into occurrence of fungus Aphanomyces frigidophilus in water of springs Dojlidy Górne, Jaroszówka and Pietrasze within the town Białystok in Podlasie Province, Poland were conducted in Winter, Spring, Summer and Autumn of the year 2005. Samples were processed in the laboratory by routine methods commonly used to isolate these organisms. Bait method with the use of hemp seeds Can...