We report here several experiences of interphase cytogenetics, using fluorescence in situ hybridization (FISH) technique, for the detection of chromosome aberrations. FISH, using alpha satellite specific probes of 18, X, Y chromosomes, was done in interphase nuclei from peripheral blood of patients with Edwards' syndrome, Klinefelter's syndrome and Turner's syndrome with healthy male and female...

In order to facilitate improved returns to research and development in African agriculture, the innovation systems approach which engenders the involvement of multiple stakeholders in its innovation pathway, has been proposed. Despite the potential of this approach, the understanding of its implementation and particularly of the process of setting up its multi-stakeholder platform is still larg...

Pericentriolar material (PCM) mediates the microtubule (MT) nucleation and anchoring activity of centrosomes. A scaffold organized by Centrosomin (Cnn) serves to ensure proper PCM architecture and functional changes in centrosome activity with each cell cycle. Here, we investigate the mechanisms that spatially restrict and temporally coordinate centrosome scaffold formation. Focusing on the mit...

A fiber’s efficiency in a short-fiber composite can be accurately solved by shear-lag methods, which can account for fiber geometry, an imperfect interface (or interphase), and extend to low volume fractions. Such an analysis was used to evaluate the aspect ratio requirements for singlewalled nanotubes (SWNT) in a polymeric composite and contrast it to conventional fibers. The aspect ratio requ...

A new three-dimensional unit cell model has been developed for modeling three constituent phases including inclusion, interphase and matrix. The total elastic modulus of nano-composite is evaluated.  Numerical results are in good agreement with the previous proposed theoretical modeling. Higher matrix and inclusion elastic modulus both can dramatically influence the total elastic modulus.

In order to describe directly interphase properties of composite, force modulation of atomic force microscopy is adopted to study the cross-section of unidirectional carbon fiber/epoxy composites systematically. Research results indicate that in force modulation mode of AFM, relative stiffness of various phases distinct in distribution, which is described by probability histogram of relative st...

Interphase centromeres are crucial domains for the proper assembly of kinetochores at the onset of mitosis. However, it is not known whether the centromere structure is under tight control during interphase. This study uses the peculiar property of the infected cell protein 0 of herpes simplex virus type 1 to induce centromeric structural damage, revealing a novel cell response triggered by cen...

Supernumerary centrosomes contribute to spindle defects and aneuploidy at mitosis, but the effects of excess centrosomes during interphase are poorly understood. In this paper, we show that interphase endothelial cells with even one extra centrosome exhibit a cascade of defects, resulting in disrupted cell migration and abnormal blood vessel sprouting. Endothelial cells with supernumerary centr...

Conventional cytogenetic is the standard technique for detection of Philadelphia (Ph) chromosome in chronic myeloid leukemia (CML). Evaluation of abelson murine leukemia/breakpoint cluster region (abl/bcr) fusion using dual-colour fluorescence in situ hybridization (D-FISH) is an alternative approach allowing rapid and reliable detection of the disease. We employed the technique of interphase D...

Computational methods can play a significant role in characterization of the carbon-based nanocomposites by providing simulation results. In this paper, we prepared a brief review of the mechanical properties of carbon nanotubes (CNTs), Graphene, and coiled carbon nanotube (CCNTs) reinforced nanocomposites. Varies simulation studies in mechanical properties of nanocomposites including represent...