PURPOSE We defined the quantitative changes in corneal epithelial nerve fibers during mouse development. METHODS Corneal whole mounts from 36 mice were immunostained with a specific anti-β-III tubulin antibody to label nerve fibers. Epithelial nerve fibers in the whole cornea were scanned and imaged using deconvolution microscopy. Subbasal nerve fiber and nerve terminal densities were calcula...

Substance P-immunoreactive nerve fibers and cell bodies were examined in the forestomach mucosa of the calf and cow pretreated with colchicine, using thick (100 microns) floating sections. Intraepithelial nerve fibers were identified, appearing only rarely in the rumen and reticulum, and completely absent from the omasum. Nerve fibers were observed in the lamina propria of all the regions of th...

Although bone is continually being remodeled and ultimately declines with aging, little is known whether similar changes occur in the sensory and sympathetic nerve fibers that innervate bone. Here, immunohistochemistry and confocal microscopy were used to examine changes in the sensory and sympathetic nerve fibers that innervate the young (10 days post-partum), adult (3 months) and aging (24 mo...

A 7-year-old girl of second degree consanguineous parents, presented with an 8 months history of intermittent exotropia of the left eye. Cycloplegic refraction was -0.25.-0.50×165o and -3.00.0.50×20o in the right and left eyes respectively. An initial objective assessment of the visual function showed a best corrected visual acuity of 12/10 in the right eye and 6/10 in the left eye. Fundoscopy ...

When denervation was performed by nerve-cutting and nerve-crushing in chickens, three phases could be observed morphologically. The first phase was characterized by foregoing degenerative changes of the neuro-muscular junctions (axon terminals), followed by atrophic changes of the muscle fibers. The second phase was characterized by irregular arrangement of the myofila-ments in atrophic muscle ...

Objective(s): The purpose of this study is to investigate the indication function of the calcium circulation-related factors on the damage to muscle strength and contraction function after nerve injury. The target factors include ryanodine receptor (RyR), inositol-1,4,5-triphosphate receptor (IP3R), phospholamban (PLN), cryptocalcitonin (CASQ), ATPase and troponin C (T...

Detection and primary processing of physical, chemical and thermal sensory stimuli by peripheral sensory nerve fibers is key to sensory perception in animals and humans. These peripheral sensory nerve fibers express a plethora of receptors and ion channel proteins which detect and initiate specific sensory stimuli. Methods are available to characterize the electrical properties of peripheral se...

STUDY DESIGN Immunohistochemical study of the sensory innervation of the human lumbar intervertebral disc. OBJECTIVE To determine the type of sensory fibers innervating human degenerated lumbar intervertebral discs. SUMMARY OF BACKGROUND DATA Sensory neurons involved in pain perception related to inflammation in rats are typically small, peptide-containing neurons immunoreactive for calcito...

The nerve fiber distribution in the oral mucosa of the soft palate and palatoglossal arch of the rat was studied by means of methylene blue supravital staining. It was focused primarily on the dye uptake of intraepithelial nerve fibers. Differences in the morphology of nerve fiber terminations were found between these regions of the oral mucosa. In the soft palate, local accumulations of intrae...