Calystegines were isolated from the root cultures of Physalis divaricata, P. pubescens, P. philadelphia,and P. philadelphia (solanaceae). Calystegines of P. divaricata were identified as calystegine A3, A5, B1 and B2, with concentrations of 6.99, 4.41, 8.52, and 14.7 ?g/g in fresh mass, respectively. Root cultures of P.pubescens contain calystegine A3, B1 and B2, P. philadelphia only contains ...

lipases are diversified enzymes in their properties and substrate specificity, which make them attractive tools for various industrial applications. in this study, an alkaline thermostable lipase producing bacteria were isolated from soil of different regions of isfahan province (iran) and its lipase was purified by ammonium sulfate precipitation and ion exchange chromatography. to select a the...

background: there are varieties of purification techniques for separation of human plasma proteins such as salting out, ion exchange chromatography, and ethanol fractionation. there are limitations for each method, for example in salting out method, the salt has to be removed in an additional step. ion exchange chromatography is difficult for scaling up, and plasma fractionation is a time consu...

Cerebrocuprein and hepatocuprein have been isolated from extracts of human brain and liver, respectively, by a method which utilizes ion exchange chromatography and gel filtration procedures. Detailed studies of the physical, chemical, and immunological properties of the purified proteins were conducted. A comparison of the results with similar data obtained for erythrocuprein from human erythr...

A manual modification of an automated method [Atkinson, K. F., Clin. Chem. 18, 1001 (1972)] for enzymatic assay of 2,3-diphosphoglycerate is described, which is suitable for small laboratories. Samples are easily prepared for analysis, and preparations are stable for several months. Virtually all of the color generated in the colorimetric assay is produced by the diphosphoglycerate-catalyzed re...

Polarity Reversed-phase chromatography and hydrophobic-interaction chromatography Centrifugation: Analytical Centrifugation. Chromatography: Protein Separation. III/Enzymes: Liquid Chromatography. Proteins: Capillary Electrophoresis; Centrifugation; Crystallization; Electrophoresis; Glycoproteins: Liquid Chromatography; High-Speed Countercurrent Chromatography; Ion Exchange; Metalloproteins: Ch...

This review summarizes the use of computer assisted liquid chromatographic method development for the analytical characterization of protein biopharmaceuticals. Several modes of chromatography including reversed-phase liquid chromatography (RPLC), ion exchange chromatography (IEX), hydrophobic interaction chromatography (HIC) and some perspectives are discussed. For all these chromatographic mo...