background and objectives: glycoprotein 96 is the primary chaperone of the endoplasmic reticulum. immunization with it induced potent cytotoxic t lymphocyte responses to intracellular bacteria. s. typhimurium is a facultative intracellular bacterium and acquired resistance against this bacterium mainly depends on activity of cytotoxic t cells. this study aimed to evaluate the capacity of glycop...

Two protein isolates (PI1 and PI2) were prepared from sunflower meal produced in Bulgaria. They were obtained by using isoelectric or ethanol precipitation following the extraction of the proteins with 10% NaCl. Their amino acid composition and water solubility as a function of pH and presence of NaCl were investigated. Lysine was established as the first limiting amino acid in the protein isol...

The tonoplast-bound ATPase of Hevea brasiliensis (caoutchouc tree) was solubilized with dichloromethan and purified 100-fold with two ammonium sulfate precipitation steps and a G-200 gel filtration step. The resulting ATPase activity eluted according to a molecular mass of approximately 200 kDa and chromatographed at an isoelectric pH of 5.3. Subunits of molecular mass 110 kDa, 68 kDa, 24 kDa a...

A procedure is described for the purification of the alpha-amanitin-sensitive DNA-dependent RNA polymerase [EC 2.7.7.6] from wheat germ. Solubilization of the enzyme activity was achieved by sonication of a crude extract in a high-salt buffer. Purification involved precipitation with protamine sulphate and (NH(4))(2)SO(4), chromatography on DEAE-cellulose and phosphocellulose, and sucrose gradi...

A vascular plasminogen activator obtained by perfusion of the vessels of the lower extremities of human cadavers has been purified to apparent homogeneity by sequential precipitation in ammonium sulfate, reverse ammonium sulfate gradient solubilization, hydrophobic chromatography on octyl-Sepharose, and gel filtration on Sephadex G-75 and G-150. The isolation procedures were carried out in the ...

The (sodium -Ipotassium)-activated adenosine triphosphatase (NaK ATPase) in Lubrol extracts of NaI-treated bovine brain microsomes has been purified 30 to 50 times over that in the microsomes by salt and isoelectric precipitation, zonal centrifugation, and a new ammonium sulfate fractionation procedure. The final step in the purification procedure renders the NaK ATPase insoluble. The partially...

This paper describes a rapid two-step procedure for the purification of the low density lipoprotein receptor from bovine adrenal cortex membranes. After solubilization with nonionic detergents, the receptor adheres tightly to a DEAE-cellulose column at pH 6. Following elution from DEAE-cellulose, detergent is removed, leaving the receptor in a soluble form. The receptor is then subjected to aff...

Failure of cholesterol homeostasis in the body can lead to cholesterol gallstone disease, the most common and costly gastrointestinal disease. The primum movens in cholesterol gallstone formation is the hypersecretion of hepatic cholesterol; this condition leads to bile chronically supersaturated with cholesterol which is prone to rapid precipitation as cholesterol crystals in the gallbladder. ...

Membrane immunoglobulin (Ig) of splenocytes and thymocytes of the goldfish, Carassius auratus, was demonstrated by indirect fluorescent-antibody techniques. Observations on shedding and resynthesis indicated that the thymocyte Ig was endogenously produced. The lymphocyte surface proteins were radioiodinated using the lactoperoxidase-catalyzed reaction, and the labeled Ig molecules were isolated...

An Arabidopsis mitochondrial proteome project was started for a comprehensive investigation of mitochondrial functions in plants. Mitochondria were prepared from Arabidopsis stems and leaves or from Arabidopsis suspension cell cultures, and the purity of the generated fractions was tested by the resolution of organellar protein complexes applying two-dimensional blue-native/N-[2-hydroxy-1,1-bis...