Previously we showed that Ani (anisodamine)/Neo (neostigmine) combination produced anti-shock effect via activating α7 nicotinic acetylcholine receptor (α7nAChR). In this study, we aim to investigate the therapeutic effect and underlying mechanisms of Ani/Neo combination in acute lethal crush syndrome (CS). In rat and rabbit CS models, Ani/Neo combination increased the 24 h survival rates, impr...

We describe a practical, technically convenient DEAE-Sephadex chromatographic column and a three-fraction salt-gradient elution procedure, with which serum creatine kinase activity is rapidly and quantitatively separated into the isoenzyme components with excellent analytical recovery of the total activity applied. The homogeneity of the isoenzyme fractions was demonstrated by electrophoresis a...

We examined the effect of storing human plasma or extracts of prostate at -90 degrees C on the activity of creatine kinase and lactate dehydrogenase and isoenzyme distribution. Enzyme activities were unaltered during storage for as long as six weeks. If these preparations were thawed only once at 2 to 4 degrees C, they could be stored for as long as 165 days at -90 degrees C with no change in i...

The topic of quality control often evokes prolific discussion among those whose business is its administration. Reliable methods for identifying mistakes must be developed in both the manufacture and use of test devices. Unit test devices are uncomplicated to run and provide quick results in a world where speed and simplicity are paramount, yet their very nature as individual assay units introd...

Creatine kinase isoenzymes from Torpedo californica electric organ, skeletal muscle, and brain were purified and characterized. Torpedo electric organ and skeletal muscle creatine kinase have identical apparent Mr, electrophoretic mobility, and cyanogen bromide fragments. The electrophoretic mobility of the Torpedo creatine kinase was anodal as compared to mammalian MM creatine kinase. No creat...

background: leishmaniasis is endemic in iran. different species of leishmania (l.) parasites are causative agents of this disease. correct identification of leishmania species is important for clinical studies, prevention, and control of the diseases. mix up of leishmania isolates is possible in the laboratory, so there is need for verification of species for isolates of uncertain identity. dif...