in this study escherichia coli de3 containing expression vector (pet21a) with cloned persian sturgeon growth hormone (psgh) gene was grown in 10 ml lb broth on a 150 rpm shaker, at the temperature of 37 °c. at the late log phase (determined by od standard curve) 100 μl isopropyl β-d-1-thiogalactopyranoside (iptg) was added for induction of gh synthesis. samples were taken every 2 hours and afte...

Isolation and separation of specific molecules is used in almost all areas of biosciences and biotechnology. Diverse procedures can be used to achieve this goal. Recently, increased attention has been paid to the development and application of magnetic separation techniques, which employ small magnetic particles. The purpose of this review paper is to summarize various methodologies, strategies...

Objective: To develop methods for the isolation and purification of round spermatids (RS) from ejaculated semen specimens recovered from patients suffering from nonobstructive azoospermia for the purpose of performing intracytoplasmic round spermatid injections (ROSI) into human oocytes. Design: Prospective study. Setting: Audrology Institute of Lexington, Lexington, Kentucky. Patients: Six men...

Lipases (E.C. 3.1.1.3) can be broadly defined as enzymes that catalyze the hydrolysis of ester bonds in substrates such as vitamin esters, phospholipids, triglycerides (TGs) and cholesteryl esters [1]. Interest in lipases has been increasing in the past few years because of the actual and potential uses of this enzyme in applications like hydrolysis of fats and oils, organic synthesis, modifica...

Cellulose is a renewable and biodegradable natural biopolymersynthesized mainly in plants such as wood, bark, cotton fiber, green algae, some bacteria and animals. Pre-treatment processes such as alkaline purification and enzymatic purification are applied in order to create a proper sorption characteristic of regenerated cellulose fiber. Prior to the application of cellulose/cellulose derivati...

There are a number of nuclear enzymes that bind nicotinamide, thymidine or nucleotides. We use this fact to prepare affinity columns for the purification of nuclear enzymes. In particular the nuclei of eukaryotic cells have an enzyme that catalyses the synthesis of the homopolymer poly(ADP-ribose) from NAD. Isolation and partial purification has been achieved (Yamada et al., 1971 ; Ueda et al.,...

A procedure was developed for the separation and purification of amylose and amylopectin isolated from cotton leaves. Cotton leaves were homogenized in 0.02 molar phosphate buffer at pH 7.0 containing HgCl(2) plus toluene. Crude starch granules were collected by centrifugation and partially purified by treating with acetone and toluene. The starch granules were then dispersed in dimethylsulfoxi...