Bioactive oxidized linoleic acid metabolites (OXLAMs) include 13- and 9-hydroxy-octadecadienoic acid (13-HODE + 9-HODE) and have been linked to oxidative stress, inflammation, and numerous pathological and physiological states. The purpose of this study was to measure changes in plasma 13-HODE + 9-HODE following a 75-km cycling bout and identify potential linkages to linoleate metabolism and es...

In vitro studies have shown that a-lipoic acid (LA) is an antioxidant. There is a paucity of studies on LA supplementation in humans. Therefore, the aim of this study was to assess the effect of oral supplementation with LA alone and in combination with a-tocopherol (AT) on measures of oxidative stress. A total of 31 healthy adults were supplemented for 2 months either with LA (600 mg/d, n 5 16...

34 Pre-eclampsia represents a state of increased or prolonged vasoconstriction, partially 35 linked to the potent vasocontractile effect of isoprostanes. The process of Rho A– 36 mediated calcium sensitization is inherent to a state of prolonged contractility in many 37 smooth muscle types. The aims of this study were 1), to investigate mRNA expression 38 levels of Rho A and Rho kinase isoforms...

Although isoprostanes generally act on smooth muscle via TXA(2)-selective prostanoid receptors (TPs), some suggest other prostanoid receptors or possibly even a novel isoprostane-selective receptor might be involved. We studied contractions to several isoprostanes in porcine pulmonary vasculature using organ bath techniques. 8-iso-prostaglandin E(2) (PGE(2)) was the most potent and efficacious ...

BACKGROUND Oxidative stress is pivotal in atherogenesis. Although the most widely used indirect assay to quantify oxidative stress is LDL oxidative susceptibility, direct assays such as urinary F(2)-isoprostanes have shown great promise. METHODS We evaluated the utility of both a direct measure of oxidative stress (urinary F(2)-isoprostanes) and an indirect measure of copper-catalyzed, LDL ox...

Introduction Cancer has been associated with increased oxidative stress and deregulation of bioactive oxylipins derived from long-chain polyunsaturated fatty acids (LC-PUFA) like arachidonic acid (AA). There is a debate whether ω-3 LC-PUFA could promote or prevent prostate tumor growth through immune modulation reduction stress. Our aim was to study the association between enzymatically non-enz...

34 Pre-eclampsia represents a state of increased or prolonged vasoconstriction, partially 35 linked to the potent vasocontractile effect of isoprostanes. The process of Rho A– 36 mediated calcium sensitization is inherent to a state of prolonged contractility in many 37 smooth muscle types. The aims of this study were 1), to investigate mRNA expression 38 levels of Rho A and Rho kinase isoforms...

In vitro and animal studies suggest that oxidative stress is associated with endothelial dysfunction. We tested whether local oxidative stress and nitric oxide (NO) bioavailability in the coronary circulation is associated with coronary endothelial dysfunction in humans. Blood samples were obtained simultaneously from the left main coronary artery and the coronary sinus for measurement of F2-is...