Rat liver glycogen was separated into fractions of uniform particle size by the rate-zonal centrifugation of homogenates through cesium chloride and sucrose density gradients. Glycogen was then isolated from other cell constituents having similar sedimentation coefficients by isopycnic-zonal centrifugation in CsCI density gradients to effect a density separation. A density of 1.62 to 1.65 was i...

Native glycogen was isolated from Tetrahymena pyriformis (HSM) by isopycnic centrifugation in cesium chloride density gradients. A density of 1.62 to 1.65 was isopycnic for glycogen. Most of the banded glycogen existed as 35 to 40 mmicro particles which had a sedimentation coefficient of 214. These particles were composed of aggregates of 2 to 3 mmicro spherical particles. Extraction of glycoge...

The major classes of lipoproteins were isolated from human plasma by ultracentrifugation in continuous density gradients using the Ti-14 and Ti-15 zonal rotors. Chylomicrons + VLDL, LDL, and HDL were separated from each other and from the more dense residual proteins (albumin fraction) of plasma by rate-zonal flotation in NaBr gradients in the density range 1.0-1.4. The chylomicron-VLDL fractio...

The isopycnic equilibrium of [131J]albumin digesting particles from mouse liver homogenates occurs, in a sucrose gradient, at a density 1.25, and it does not change with time after albumin injection (5 to 300 minutes).

There are a number of barriers to the adoption of improved parameterizations of diapycnal mixing in large-scale ocean models. Two such barriers are discussed in detail. Ocean models often exhibit levels of spurious numerical diapycnal mixing that are so large, compared with the physical values, that parameterizing additional mixing is counterproductive, even when that additional mixing is physi...

Sedimentation velocity was measured in a Spinco ultracentrifuge’ operating at 165,000 X g. The hormone solutions (0.5 per cent) were prepared with borate buffer of pH 9.7 or saline at pH 9.1. Table I presents a typical run in a borate buffer; the sedimentation velocity constant was calculated by the procedure described by Svedberg and Pedersen (3). It may be noted that the individual values of ...