Anopheles superpictus Grassi is one of the most widespread malaria vectors in Iran. Two morphologically independent forms of this mosquito, both at larval and adult stage as well as a great diversity in its mtDNA loci have been previously described in Iran. Because of existence of mtDNA haplotypes, co-occurrence of two forms in diverse climates as well as different roles of populations played i...

Small liver and minute intestinal flukes are highly prevalent in Southeast Asia, and in mixed infections, their eggs are difficult to differentiate morphologically in fecal samples. PCR assays targeting the ITS regions in ribosomal DNA were designed to identify and differentiate species. The PCR amplicons of Opisthorchis viverrini, Clonorchis sinensis, Haplorchis pumilio, and Haplorchis taichui...

PREMISE OF THE STUDY DNA metabarcoding has broad-ranging applications in ecology, aerobiology, biosecurity, and forensics. A bioinformatics pipeline has recently been published for identification using a comprehensive database of ITS2, one of the common plant DNA barcoding markers. There is, however, no corresponding database for rbcL, the other primary marker used in plants. METHODS Using pu...

Small ribosomal subunits are co-transcriptionally assembled on the nascent precursor rRNA in Saccharomyces cerevisiae. It is unknown how the highly intertwined structure of 60S large ribosomal subunits is initially formed. Here, we affinity purified and analyzed a series of pre-60S particles assembled in vivo on plasmid-encoded pre-rRNA fragments of increasing lengths, revealing a spatiotempora...

We evaluated a combined panfungal PCR-reverse line blot (RLB) hybridization assay based on internal transcribed spacer 1 (ITS1) and ITS2 region polymorphisms to identify 159 Candida, Cryptococcus neoformans, and Aspergillus isolates (22 species). Its utility to identify fungal pathogens directly from 27 clinical specimens was also determined. ITS sequence analysis was performed to resolve discr...

30 milled rice samples were collected from retailers in four states of Malaysia. These samples were evaluated for Eurotium spp. contaminations by direct plating on malt extract salt agar (MESA). All Eurotium were isolated and identified based on morphology and nucleotide sequences of internal transcribed spacer 1 (ITS1) and ITS2 of the rDNA.  Four Eurotium species (E. rubrum, E. amstelodami, E....

OBJECTIVE To identify the etiologic agent from a paragonimiasis patient using molecular techniques. METHODS The complete nuclear ribosomal DNA second internal transcribed spacer (ITS2) gene sequence of eggs in sputum from a paragonimiasis patient was obtained by directly auto-sequencing its PCR product. ITS2 genes from eggs of Paragonimus westermani and Pagumogonimus skrjabini (both from anim...

We sequenced the internal transcribed spacer 2 of the ribosomal DNA (ITS2-DNAr) from the three Schistosoma mansoni intermediate hosts in Brazil: Biomphalaria glabrata, Biomphalaria tenagophila and Biomphalaria straminea. Analysis of a restriction map from those sequences allowed us to select putative restriction enzymes able to identify the snail species under study. Four restriction enzymes we...

The aim of this study was to investigate the level of evolutionary divergence among five isofemale lineages of Metopolophium dirhodum Walker (Hemiptera: Aphididae) using sequences of the Internal Transcribed Spacer (ITS) of ribosomal DNA (rDNA). These isofemalelineages were derived from parthenogenetic females, previously genetically characterized by having strong association of RAPD (Random Am...

The diversity of endosymbiotic dinoflagellates (Symbiodinium) in corals at Johnston Atoll in the central Pacific Ocean was assessed using both the internal transcribed spacer 2 (ITS2) region of the nuclear rDNA and chloroplast 23S rDNA. More sequences were recovered from corals using the ITS2 primers than with the chloroplast 23S primers, a finding that reflects both the higher taxonomic resolu...