Name Type Company Catalog Number Comments Dithizone reagent Dissolve 5mg Dithizone in 5 ml of DMSO in a 50 ml conical tube. Add 45 ml of normal saline and mix gently. Filter with a 60 cc syringe and a 0.22 "m syringe filter into a new 50 ml tube. Store at 4 OC, until use. Add 1 ml to each 35 mm counting Grid-plate, when needed. PBS Buffer HBSS Buffer Isolation soln. Buffer HBSS containing 10 mM...

As in all fluorometric procedures, glassware, reagents, and specimens must be kept free of fluorescent contamination. Glassware must be cleaned with an inorganic detergent and rinsed with distilled water. Paraflim should be used to seal tubes for mixing procedures. Ten milliliters of venous blood was collected into tubes containing 0.1 ml. sodium citrate (38 gm./100 ml. water), or 20 mg. ethyle...