There are available results of electron microscopic studies as. regards the oral mucosa by such researchers as Sognnaes and Albright (1956, 1958), Themann (1958), Fasske and Themann (1959), Frenkel (1959), Albright (1960), Rhodin and R eith (1962) and Arai (1961, 1962). But all of them fail to give a description of the epithelium of the tongue, and only Rhodin and R e i t h give a brief mention...

Artificial skin or skin equivalents have been used for clinical purpose to skin graft and as substitutes for animal experiments. The culture of cell lines such as HaCaT has the potential to produce large amounts of artificial skin at a low cost. However, there is a limit to keratinization due to the restriction of differentiation in HaCaT. In this study, a culture device that mimics the in vivo...

Porokeratosis (PK) is an uncommon disorder of epidermal keratinization characterized by annular plaques with an atrophic center surrounded by a raised, keratotic wall, with unknown aetiology and an unpredictable outcome. It has several clinical forms including porokeratosis of Mibelli, giant porokeratosis, linear porokeratosis, disseminated superficial actinic porokeratosis, palmoplantar ...

Keratinized mucosa is of fundamental importance to maintain healthy gingival tissue, and understanding the mechanisms of oral mucosa keratinization is crucial to successfully manage healthy gingiva. Previous studies have shown a strong involvement of the basement membrane in the proliferation and differentiation of epithelial cells. Therefore, first, to identify the keratinized mucosa-specific ...

Several reports have suggested that mesenchymal glycosaminoglycans (GAG) may be involved in the regulatory role of epithelial differentiation. Some researchers have pointed out that exogenous GAG affects extracellular GAG accumulation. We have therefore examined the effect of added GAG on two typical processes of avian skin differentiation: keratinization and feather formation. Glycosaminoglyca...

Leukoplakias are oral lesions that may have many clinical and histological aspects and they are usually associated with malignancy when dysplastic alterations are shown. However, these transformations may occur in non-dysplastic lesions that show harmless clinical aspect. For this reason, the proposal was to study the p53 and PCNA immunohistochemical expression in non-dysplastic leukoplakias, t...

Porokeratosis of Mibelli is a keratinization disorder due to cellular clones that show different degress of dysplasia. Sometimes, these lesions develop on the previously traumatized or burnt skin. Herein, a 24 – year- old female patient is presented with three facial skin lesions on her right cheek on a previous old scar due to cutaneous leishmaniasis is presented. The larger lesion was an eryt...

The Nara Bladder Tumor No. 2 cell line, established from a urinary bladder carcinoma in the Wistar rat, formed keratinizing cells and multicellular pearls in meniscus gradient culture when fed with a medium of 15 or 30% fetal calf serum in Eagle's minimal essential medium. Confluent monolayer cultures were first prepared with the tubes in conventional horizontal position. When cultures were the...