L-asparaginase (L-asparagine amido hydrolase, E.C.3.5.1.1) is a promising chemotherapeutic agent which plays a vital role in treatment of a variety of lymphoproliferative disorders, lymphosarcoma and acute lymphoblastic leukemia in particular. An attempt is made in the present study to optimize the production of L-asparaginase by Fusarium equiseti using soya bean meal under solid state fermenta...

L-asparaginase (L-asparagine amido hydrolase, E.C.3.5.1.1) is a promising chemotherapeutic agent which plays a vital role in treatment of a variety of lymphoproliferative disorders, lymphosarcoma and acute lymphoblastic leukemia in particular. An attempt is made in the present study to optimize the production of L-asparaginase by Fusarium equiseti using soya bean meal under solid state fermenta...

Crystalline glutaminase-asparaginase which is effective against solid as well as ascites tumors was prepared from soil isolate organism Pseudomonas 7A. This enzyme has a ration of Vmax for L-glutamine and L-asparagine of 2.0. The presence of glutamic acid in the growth medium is essential for optimal enzyme production and glucose inhibits the production of glutaminase-asparaginase. The purifica...

A species of Streptomyces, Streptomyces ginsengisoli, a river isolate, was evaluated for production of an enzyme, L-asparaginase, with multiple functions mainly anticancer activity. The actinomycete was subjected to submerged fermentation by "shake flask" method. The quantity of L-asparaginase produced was estimated as 3.23 μ mol/mL/min. The effect of various culture conditions on L-asparaginas...

SUMMARY The bone marrow of patients with acute lymphocytic leukemia, acute myelogenous leukemia, and metastatic cancer (normal marrow) was evaluated for L-asparagine dependence with the use of the in vitro test of Sobin and Kidd. All of the marrows including the normal ones showed L-asparagine dependence. There was a direct correlation between the concentration of L-asparaginase and depression ...

The enzyme L-asparaginase has been proved to be efficient in treatment of Acute Lymphoblastic Leukemia [ALL] and Lymphosarcomas. The present paper discusses the studies carried out for the production of this enzyme from bacillus species. By study obtained optimization of medium, solid state fermentation by using cotton cake and wheat bran and submerged fermentation using distillery waste. a M2 ...

Asparaginases are known to be the cornerstone for treatment of acute lymphoblastic leukemia (ALL) and are used for treatment in all pediatric regimens as well as in the majority of adult treatment protocols. Clinical hypersensitivity reactions against commercially available asparaginase have resulted in failure of asparaginase in treatment of ALL in more than 60% of cases. Thus, it is required ...

in the present study, streptomyces gulbargensis and its mutant form, s.gulbargensis mu24, immobilized on polyurethane foam were investigated for the production of l-asparaginase using groundnut cake extract as medium. the medium with an initial ph of 8.5 was inoculated with free and immobilized cells separately and then subjected to fermentation by incubation at 40oc and shaking at 200 rev/min....

In plants, ammonium is first assimilated into the amino acids L-Gln and L-glutamate and then channeled into L-aspartate and L-Asn. These amino acids serve as nitrogen donors in biosynthetic reactions, as well as compounds for intercellular nitrogen transport. In amide-transporting plants, Asn can act as the sole nitrogen source for the growth of plant tissues. In higher plants the catabolism of...

L-Asparaginase enzyme has received great attention owing to its potential as anticancer drug and food processing agent. L-Asparaginase producing microbes are conventionally screened on phenol red plates containing L-asparagine as the sole nitrogen source for its growth. However, the contrast of the zone in phenol red plates is not very distinct. To overcome this problem, an alternate methodolog...