Cryptococcus neoformans laccase expression during murine infection was investigated in lung tissue by immunohistochemistry and immunogold electron microscopy. Laccase was detected in the fungal cell cytoplasm, cell wall, and capsule in vivo. The amount of laccase found in different sites varied as a function of the time of infection.

A comparison of Pycnoporus cinnabarinus strains for laccase production was carried out. A dikaryotic strain, I-937 strain, producing a high level of laccase (9500 U l(-1)) was selected. The study of the life cycle in vitro of this dikaryotic strain led to isolation of monokaryons. Forty-eight monokaryotic strains were isolated and screened for laccase production. One of these strains, ss3, prod...

GOCHEV, V. K. and A. I. KRASTANOV, 2007. Isolation of laccase producing Trichoderma spp. Bulg. J. Agric. Sci., 13: 171-176 Based on its dye decolorization activities four Trichoderma strains were screened as laccase producers. The strains were identified as T. atroviride, T. longibrachiatum, T. reesei, and T. viride. It was determined that it was expedient to screen laccase producing Trichoderm...

Fungal laccase can be detected with the nonautooxidizable laccase­ specific compound, syringaldazine, in the presence of tyrosinase and per­ oxidase. Data are presented from tests with the azine derivative of numerous fungous sporophores and associated decayed wood tissues from various localities in Wisconsin and Oregon. Syringaldazine is shown to be a reliable replacement and more specific rea...

The oxidation of naphthalene by immobilized laccase from Trametes versicolor has been performed using diverse immobilization strategies on mesostructured silica materials. Laccase was immobilized by physical adsorption on several SBA-15 with different textural properties and by covalent attachment on functionalized SBA-15 prepared by co-condensation method (direct synthesis). The adsorption of ...

Five white-rot basidiomycetes (WRB) species have been evaluated for their potential to tolerate and to degrade 0.2 mM 2, 4, 6-trinitrotoluene (TNT) as well as to produce laccase and manganese peroxidase (MnP) in presence of this xenobiotic. The tested fungal strains produced laccase in both glycerol and mandarin peels-containing media, whereas in the glycerol-containing medium only Cerrena unic...

Catechin was oligomerized using free laccase and laccase-gum Arabic conjugate. The process of oligomerization was optimized with respect to solvent, ratio of solvent to buffer (0.2:10 to 1:10), pH of buffer (3-10), enzyme (575-18,400 U/mg) and substrate concentration (1-7mM). Maximum production of oligomer was observed in methanol at ratio 0.6:10 of methanol:buffer of pH 5 using 2300 U/mg of la...

When laccases are applied for detoxifying steam-exploded slurries, enzymatic hydrolysis is affected. In this study, 10% (w/v) of dry weight (DW) slurry or water insoluble solid fraction were subjected to 48 h enzymatic hydrolysis in the presence of different laccase loadings (0.1, 0.5, 5 or 10 IU/g DW). All laccase dosages higher than 0.1 IU/g DW significantly inhibited glucose recovery. Nevert...

A highly efficient and stable biocatalyst (denoted D201_Lac) was fabricated by encapsulating Trametes versicolor laccase within a macroporous and strongly basic exchange resin D201 through a simple adsorption process. Transmission electron micrographs and Fourier transform infrared spectra of the resultant D201_Lac proved that nanosized laccase clusters were embedded into the inner nano-pores/c...

Laccase from Pleurotus florida was immobilized on agarose/agar/polyacrylamide gel, glass beads and alginate beads. The laccase entrapped agarose gel exhibited 88% of immobilization yield as compared to laccase immobilization on glass bead (72%) and Ba-alginate (50%). The enzyme embodied in agarose gel showed greater storage stability at 16°C as compared to enzyme immobilized on glass beads and ...