OBJECTIVES To characterize the chromosome-encoded metallo-β-lactamase (MBL) from the psychrophilic, marine fish-pathogenic bacterium Aliivibrio salmonicida LFI1238 and check for the presence of the gene in other Aliivibrio isolates both connected to the fish-farming industry and from the environment. METHODS The MBL gene was cloned and intracellularly expressed in Escherichia coli. Kinetic pa...

Rapid detection of metallo-beta-lactamase (MBL)-producing gram-negative pathogens is critical to prevent their widespread dissemination. Thus far, no standardized phenotypic method is available, and previously reported techniques have poor sensitivity for detecting carbapenem-susceptible MBL-carrying isolates, an increasingly described phenomenon. We developed a phenotypic detection method usin...

BACKGROUND To investigate the presence of metallo-β-lactamase (MBL) genes and the genetic environment of the New Delhi metallo-β-lactamase gene bla(NDM-1) in bacteria of food animal origin. METHODOLOGY/PRINCIPAL FINDINGS Gram-negative bacteria with low susceptibility to imipenem (MIC>8 µg/mL) were isolated from swab samples collected from 15 animal farms and one slaughterhouse in eastern Chin...

INTRODUCTION: We compared the prevalence and antimicrobial susceptibility of non-fermenting gram-negative bacilli (NFGNB) isolated from clinical samples at a Brazilian tertiary care hospital in 2008 and 2013. METHODS: Collected data included patient's name, age, sex, inpatient unit, laboratory record number, type of biological material, culture test result, and antimicrobial susceptibility of...

Current study was conducted to determine prevalence and antimicrobial susceptibility patterns, ESBL, AmpC and carbapenemase- producers among clinical isolates of Enterobacteriaceae. Three hundred and seven non-duplicative clinical isolates were collected from hospitalized patients in five medical centers in Azerbaijan, Iran. Initial screening for β-lactamase production was performed using disc ...

background and objective: metallo-beta-lactamase (mbl)-mediated resistance is an emerging threat in hospital isolates of pseudomonas aeruginosa. there is not enough information from iran regarding the prevalence and the screening methods for such enzymes. the present study was undertaken to detect metallo betalactamase in strains of p. aeruginosa isolated from burned patientusingphenotypic meth...

Metallo-β-lactamase-producing Pseudomonas aeruginosa (MPPA) is an important nosocomial pathogen that shows resistance to all β-lactam antibiotics except monobactams. There are various types of metallo-β-lactamases (MBLs) in carbapenem-resistant P. aeruginosa including Imipenemase (IMP), Verona integron-encoded metallo-β-lactamase (VIM), Sao Paulo metallo-β-lactamase (SPM), Germany imipenemase (...

In eukaryotes, the process of messenger RNA 3'-end formation involves endonucleolytic cleavage of the transcript followed by synthesis of the poly(A) tail. The complex machinery involved in this maturation process contains two proteins of the metallo-beta-lactamase (MBL) superfamily, the 73 and 100 kDa subunits of the cleavage and polyadenylation specificity factor (CPSF). By using an in vitro ...

The surveillance of Device associated infections in ICUs can reduce incidence of Nosocomial infections (NIs), healthcare cost & a better control of infections. Aims & Objectives: To know the occurrence of device associated infection rate in ICU and to know the bacterial profile of various devices associated infections. Materials and Methods: This study was conducted between October 2011 to May ...

Introduction: Acinetobacter baumannii is one of the major pathogens causing nosocomial infections due to emergence of resistance to various antimicrobial agents. Resistance due to antimicrobial degrading enzymes is now a worldwide problem and a major reason of concern for the treating physicians. Keeping this in mind, the present study was designed to isolate Acinetobacter baumannii and study v...