The aim of this study was to analyze the usefulness of fecal lactoferrin in the diagnosis and monitoring of inflammatory bowel disease (IBD) in children. The study included 52 children with IBD (24 with Crohn's disease and 28 with ulcerative colitis) aged between 0.92 and 18 years, and 41 IBD-free controls of similar age. Fecal concentration of lactoferrin was determined with a quantitative imm...

Lactoferrin is a major constituent of polymorphonuclear leukocyte granules and is present in mature neutrophils but not in blasts or promyelocytes. We have isolated a cDNA probe for lactoferrin and used it to study the synthesis of lactoferrin mRNA by normal and leukemic granulocyte precursors. The probe pHL-41 has been subcloned in phage m13 and characterized by restriction endonuclease analys...

We compared the susceptibility to viral infection of splenocytes, isolated from young versus old CBA mice, and evaluated the antiviral actions of lactoferrin in splenocytes infected with Encephalomyocarditis virus (EMCV). Recombinant mouse lactoferrin (rmLF) and bovine lactoferrin (bLF) were used. There were no differences in the susceptibility to EMCV infection in the studied age categories. B...

s of the 7th International Conference on Lactoferrin / Résumés de la 7e Conférence internationale sur la lactoferrine N. Kumagai, S. Tsunematsu, H. Hamano, K. Yamauchi, and K. Tsuchimoto 385 Oral administration of lactoferrin enhances viral decline in patients with genotype-1B-related chronic hepatitis C treated with Peginterferon a-2B plus ribavirin T. Kuhara, K. Yamauchi, and M. Takase 385 Or...

BACKGROUND Although elevated levels of lactoferrin provide a biomarker for inflammatory bowel diseases and colorectal cancer, the clinical significance of these elevated levels in ascitic fluid of patients with ascites caused by liver cirrhosis is limited. The aims of our study were to investigate the usefulness of ascitic fluid lactoferrin levels for the diagnosis of spontaneous bacterial peri...

Lactoferrin is a versatile protein present in most bodily fluids of mammals. Because more and important functions lactoferrin the human body are being discovered, interest its industrial production application consumer goods increasing accordingly. Today, primarily isolated from bovine milk or sweet whey using cation exchange column chromatography – costly but efficient high-throughput process ...

We read with interest the report of Guerrant et al. (1). It was stated that the fecal lactoferrin assay may be a useful diagnostic tool in the evaluation of patients with acute diarrhea syndrome (ADS), offering some advantages over microscopic examination for fecal leukocytes. We would like to comment on this and report data from a study carried out in Chile in which fecal lactoferrin was measu...

Neutrophil specific granule deficiency (SGD) is a congenital disorder associated with an impaired inflammatory response and a deficiency of several granule proteins. The underlying abnormality causing the deficiencies is unknown. We examined mRNA transcription and protein synthesis of two neutrophil granule proteins, lactoferrin and myeloperoxidase in SGD. Metabolically labeled SGD nucleated ma...

Lactoferrin, a multifunctional iron binding glycoprotein, plays an important role in immune regulation and defence mechanisms against bacteria, fungi and viruses. Lactoferrin's iron withholding ability is related to inhibition of microbial growth as well as to modulation of motility, aggregation and biofilm formation of pathogenic bacteria. Independently of iron binding capability, lactoferrin ...

Lactoferrin is a non-heme iron-binding glycoprotein, produced by mucosal epithelial cells and granulocytes in most mammalian species. It is involved in regulation of immune responses, possesses anti-oxidant, anti-carcinogenic, anti-inflammatory properties, and provides protection against various microbial infections. In addition, lactoferrin has been implicated in protection against the develop...