biotinylated antibody bound to streptavidin-coated donor beads and a second antibody conjugated to AlphaLISA acceptor beads. The binding of the two antibodies to the analyte brings donor and acceptor beads into proximity. Laser irradiation of donor beads at 680 nm generates a flow of singlet oxygen, triggering a cascade of chemical events in nearby acceptor beads, which results in a chemilumine...

In conjunctionwith the increasing availability of cost-efficient laser units during the recent years, laser-based micromachining techniques have been developed as an indispensable industrial instrument of ‘‘tool-free’’ high-precision manufacturing techniques for the production of miniaturized devices made of nearly every type of materials. Laser cutting and drilling, as well as surface etching,...

In the recent years, WiFi standard has been used to develop different kind of wireless networks due to its flexibility and the availability of cheap off the shelf hardware. Even if the standard itself lacks mobility support, it has been used in networks with mobile nodes. When mobility is involved, a fast handoff is of paramount importance, especially with multimedia applications. The current I...

Necessity spin-offs are organized by employees of incumbent firms to escape deteriorating job conditions. This paper proposes a conceptual model of the spin-off process. Necessity spin-offs are distinguished from opportunity spin-offs on the basis of their triggering events. An empirical analysis of German laser spin-offs traces differences in the performance and determinants of the two types o...

We demonstrate a temporal imaging system based on parametric mixing that allows simple triggering from an external clock by using a time-lens-based pump laser. We integrate our temporal imaging system into a time-to-frequency measurement scheme and demonstrate the ability to perform characterization of temporal waveforms with 1.4-ps resolution and a 530-ps record length. We also integrate our s...

This paper presents our approach for laser-based local position tracking based on the data explored in a three-dimensional environmental model of an indoor environment. This algorithm is used to substitute the dead reckoning on a mobile robot to allow robust map generation and position dependent task triggering. The underlying concept of the local environmental model used for filtering of the s...

We present an experimental study of the transition to turbulence in a plane Poiseuille flow. Using a well-controlled perturbation, we analyze the flow by using extensive particle image velocimetry and flow visualization (using laser-induced fluorescence) measurements, and use the deformation of the mean velocity profile as a criterion to characterize the state of the flow. From a large parametr...

UNLABELLED We have recently described that local contractions can be triggered by a preceding twitch in rat cardiac trabeculae in or near damaged ends of the muscle. These local contractions appeared to propagate along the trabeculae with a constant velocity; hence, they can be denoted as triggered propagated contractions (TPCs). These studies have also shown that stretch and/or subsequent shor...

Using a standard confocal setup, a UV ablation method can be utilized to selectively induce cellular injury and to visualize single-cell responses and cell-cell interactions in the CNS in real-time. Previously, studying these cell-specific responses after injury often required complicated setups or the transfer of cells or animals into different, non-physiological environments, confounding imme...