Background: Caspase-3 is involved in the execution of apoptosis and widely used as an apoptotic marker. Tumor necrosis factor-α (TNF-α) released from activated macrophages has various functions such modulation cell growth differentiation, immunoregulation, coagulation, regulation endothelial function. This study investigated immunohistochemical staining caspase-3 TNF-α expression placentas preg...

the leukotrienes and prostaglandins are biologically active metabolites derived from arachidonic acid. the leukotrienes have a role in inflammatory diseases such as allergic rhinitis, inflammatory bowel disease and asthma. montelukast, a cysteinyl leukotriene receptor antagonist, is claimed to be effective in asthma. the present study aimed to assess the role of cysteinyl leukotriene receptor a...

oxidative stress mediated by reactive oxygen species is known to contribute to the inflammatory process of bronchial asthma. reactive oxygen species are released into the bronchial tree by activated inflammatory cells. in this study, we aimed to determine the effect of vitamin c administration on leukocyte vitamin c level as well as severity of asthma. in this double blind clinical trial study ...

We designed a 48-well chemotaxis chamber to minimize manipulation time and amount of material required by the larger blindwell or Boyden chemotaxis chamber. Cell and chemoattractant dose-response curves showed that results were comparable to our better than those obtained with blindwell chambers. The volume of chemoattractant per well is 25 microliter; the number of cells can be as low as 10,00...

This study has investigated the chemotactic activity of polymorphonuclear cells (PMNs) isolated from the blood of patients with either articular rheumatoid arthritis (RA) or RA with extra-articular manifestations. A double fluorochrome immunofluorescent staining test has been employed to identify cell-associated immunoglobulins, probably immune complexes. The results suggest an inverse relation...

Purified Pseudomonas aeruginosa elastase cleaved human type III and IV collagens with the formation of specific cleavage products. Furthermore, type I collagen appeared to be slowly cleaved by both P. aeruginosa elastase and alkaline protease. These cleavage fragments from type III and IV collagens were separated from the intact collagen chains by SDS polyacrylamide gradient gel electrophoresis...

Cryopreservation of peripheral blood leukocytes is widely used to preserve cells for immune response evaluations in clinical trials and offers many advantages for ease and standardization of immunological assessments, but detrimental effects of this process have been observed on some cell subsets, such as granulocytes, B cells, and dendritic cells. Assaying fresh leukocytes gives a more accurat...

conclusions systemic arterial leukocyte filtration reduces the intubation time but has no other beneficial effect on the outcome of patients undergoing cabg or valve surgery. results the postoperative intubation time was significantly shorter in patients with leukocyte filters (0.014). there was no significant difference between two groups regarding other outcome relatedvariables. patients and ...

Aim: The purpose of this prospective cohort study is to evaluate the possible microbiome amniotic cavity in second trimester asymptomatic pregnant women by culture-based technique. Materials and Methods: This was conducted Gaziantep University Gynecology Obstetrics Clinic between October 2017 November 2019. 100 who had amniocentesis for genetic screening fetus, no complaints or clinical symptom...

Background Increasing evidence in recent years highlights the predictive and prognostic importance of expression DNA repair proteins cancer treatment. Generally, western-blotting or immunohistochemical staining methods are often used to determine proteins. These might cause misleading results such as binding nonspecific molecules by cross-reaction false negativity a result inability antibodies ...