TWIK-related acid-sensitive potassium channels (TASKs)-like current was recorded in orexin neurons the lateral hypothalamus (LH), which are essential respiratory chemoreflex. However, specific mechanism responsible for pH-sensitivity remains elusive. Thus, we hypothesized that TASKs contribute to In present study, found TASK1 and TASK3 were expressed neurons. Blocking or microinjecting acid art...

The present study was designed to examine the effect of intracerebroventricular (icv) administration of antilipopolysaccharide (LPS) antibody and blockade of Toll-like receptor 4 (TLR4) during immune stress induced by intravenous (iv) LPS injection on the gonadotropin-releasing hormone/luteinizing hormone (GnRH/LH) secretion in anestrous ewes. Injection of anti-LPS antibody and TLR4 blockade si...

Glutamate and its receptor agonists, NMDA, AMPA, and KA, elicit feeding when microinjected into the lateral hypothalamus (LH) of satiated rats. However, determining the relative contributions of AMPA receptors (AMPARs) and KA receptors (KARs) to LH feeding mechanisms has been difficult due to a lack of receptor selective agonists and antagonists. Furthermore, LH injection of KA produces behavio...

The luteinizing hormone (LH) receptor plays a pivotal role in reproduction. The high-molecular-weight (HMW) human chorionic gonadotropin (hCG) and LH are the endogenous ligands of this receptor and bind to its large N terminus. The present study characterizes the binding of a new low-molecular-weight (LMW) radioligand, [(3)H]5-amino-2-methylsulfanyl-4-[3-(2-morpholin-4-yl-acetylamino)-phenyl]-t...

The quantitative relationship between receptor binding and hormone secretion at the single-cell level was investigated in the present study by combining a reverse hemolytic plaque assay for measurement of luteinizing hormone (LH) secretion from individual pituitary cells with an autoradiographic assay of 125I-labeled gonadotropin-releasing hormone (GnRH) agonist binding to the same cells. In th...

introduction: previous studies have shown that stimulation of lateral hypothalamus (lh) produces antinociception. orexin-a (oxa) receptor is strongly expressed in the nucleus locus coeruleus (lc) and orexinergic fibers densely project from lh to lc. in this study, we assessed the role of lc and its oxa receptors in antinociceptive response induced by lh chemical stimulation in the rat. methods:...

As the expression of the LH receptor (LH-R) in granulosa cells is thought to be associated with later stages of folliculogenesis, this study was undertaken to evaluate the presence of LH-R mRNA as a suitable marker for developmental competence of oocytes. Granulosa cells and cumulus-oocyte complexes (COCs) were recovered from cows that had received ovarian stimulation. The COCs were subjected t...

Arif Siddiqui, Saeeda Shaharyar Department of Biological and Biomedical Sciences, The Aga Khan University, Karachi. Pakistan Background: The present study was designed to inves tigate the role of the 5-HT7 receptors in lordosis and release of LH and compare the lordotic responses with 5-HT1A agent. Methods: Ovariectomised but oestradiol benzoate (OB) (10 ug) for 48 h plus by progesterone (0.5 m...

The luteinizing hormone-human chorionic gonadotropin (LH-hCG) receptor was isolated from 8-liter batches of a supernatant (7,000 X g) of bovine corpora lutea homogenate from 1,200 ovaries obtained biweekly from the Wampole Laboratories, Princeton, NJ. The supernatant was concentrated by ultrafiltration to remove an inhibitor of hormone-receptor binding and inert proteins of M, < 50,000. The con...