Over the years, fluorescence microscopy has evolved and become a necessary element of life science studies. Microscopy elucidated biological processes in live cells organisms, also enabled tracking biomolecules real time. Development highly sensitive photodetectors light sources, addition to evolution various illumination methods fluorophores, helped acquire single-molecule sensitivity, enablin...

Light microscopy has long been an indispensable tool for cell biology research. From biological problems to biological knowledge, there are two more critical links in the light microscopy approach: labeling and quantitative analysis. Therefore, an integrative approach is desirable in order to deal with practical challenges in biological light microscopy.