Ku is an ubiquitous nuclear heterodimeric protein consisting of p70 and p86 subunits that binds double-stranded DNA termini and associates with chromosomes in vivo. It was originally described as an autoantigen in patients with certain autoimmune diseases. The individual subunits of Ku have been difficult to isolate from human cells without denaturation and attempts to produce functional recomb...

The alpha1- and beta1-subunits of human soluble guanylate cyclase (sGC) were coexpressed in the Sf9 cells/baculovirus system. In addition to the native enzyme, constructs with hexahistidine tag at the amino and carboxyl termini of each subunit were coexpressed. This permitted the rapid and efficient purification of active recombinant enzyme on a nickel-affinity column. The enzyme has one heme p...

Abu Ali Hossein Ebn Abdullah Ben Ali Ben Sinna entitled as Shayhk-o-Rayeis in the East and well known in the west by the name of Avicenna was born in a place called Afsharneh near the city of Bokhara in 980 AD but resided in the city of Balkh. He was five years old when his father took him to the city of Bokhara to study Holy Quran where his extraordinary memory became apparent and he memorized...

Abu Ali Hossein Ebn Abdullah Ben Ali Ben Sinna entitled as Shayhk-o-Rayeis in the East and well known in the west by the name of Avicenna was born in a place called Afsharneh near the city of Bokhara in 980 AD but resided in the city of Balkh. He was five years old when his father took him to the city of Bokhara to study Holy Quran where his extraordinary memory became apparent and he memorized...

BACKGROUND A versatile strategy for protein-surface coupling in biochips exploits the affinity for polyhistidine of the nitrilotriacetic acid (NTA) group loaded with Ni(II). Methods based on optical reflectivity measurements such as spectroscopic ellipsometry (SE) allow for label-free, non-invasive monitoring of molecule adsorption/desorption at surfaces. RESULTS This paper describes a SE stu...

Here we describe a generic procedure for the expression and purification of milligram quantities of functional recombinant eukaryotic integral membrane proteins, exemplified by hexahistidine-tagged bovine rhodopsin. These quantities were obtained with the recombinant baculovirus/Sf9 insect cell-based expression system in large-scale bioreactor cultures with the use of a serum-free and protein-f...

The crystal structure of a mutant form of the single-chain fragment (scFv), derived from the monoclonal anti-His tag antibody 3D5, in complex with a hexahistidine peptide has been determined at 2.7 A resolution. The peptide binds to a deep pocket formed at the interface of the variable domains of the light and the heavy chain, mainly through hydrophobic interaction to aromatic residues and hydr...