One of the most extensively utilized rapid characterization, release and stability testing methods therapeutic proteins in biopharmaceutical field today is capillary SDS gel electrophoresis using borate cross-linked high molecular weight dextran. In spite its widespread use, however, composition dependent separation characteristics this very unique sieving matrix has not been investigated yet. ...

In this paper, the effect of chitosan molecular weight on the characteristics (size, encapsulation efficiency, zeta potential, surface morphology and release rate) of vitamin C encapsulated tripolyphosphate cross-linked chitosan (TPP-chitosan) microspheres. The molecular weight of chitosan had a noticeable influence on the size, encapsulation efficiency, zeta potential, surface morphology and c...

Dextran, a family of natural polysaccharides, consists of an α (1→6) linked-glucose main (backbone) chain having a number of branches. The determination of the types and the quantities of branches in dextran is important in understanding its various biological roles. In this study, a hyphenated method using high-performance anion exchange chromatography (HPAEC) in parallel with pulsed amperomet...

Hydration of cross-linked starch microspheres, commercially available as a medical device, was investigated using a multi-method approach. We found that the uptake of water is accompanied by substantial swelling and changes of the polymer structure. Sorption calorimetry provided information about thermodynamics of water sorption, revealed presence of isothermal glass transition and absence of h...

shown by demonstrating that the overall rate of dextransucrase synthesis is independent of the concentration of 1,6-linked dextran acceptor if synthesis is continued for periods that are directly proportional to the amount of acceptor present. For example, in the presence of 1 mg of acceptor dextran, the rate of dextransucrase action measured over lOmin is identical with the rate measured over ...

in the present study, the production of dextran from date extract and sucrose as carbon sources by the bacterium leuconostoc mesenteroides nrrl b512 (f) was investigated. in comparison to blue dextran (mw≈2000 da), dextran molecular weight was reduced (mw2000 kda). flow behavior indices of dextran solutions from date extracts were investigated and rheological parameters were evaluated at concen...

In this study suitable, smooth, spherical, cross-linked egg albumin microspheres in the size range of 15-40 um loaded with norethisterone were prepared by multiple emulsion (o/w/o) method by the glutarldehyde cross-linking and thermal denaturation technique. Microspheres were prepared by using different drug/polymer ratios. Egg albumin was used as a mucoadhesive polymer in the formulation to in...

The objective of this study was to formulate and evaluate the drug-polymer interaction of mefenamic acid (MA) using two polymers with different characteristics as ethylcellulose (EC) and/or cellulose acetate phthalate (CAP). Microspheres were prepared by the modified emulsion solvent evaporation (MESE). The effect of drug-polymer interaction was studied for each of microspheres. Important param...

Also described as low viscous or low viscosity dextran, dextran 40 is prepared by acid hydrolysis and differential fractionation of a crude macromolecular polysaccharide produced from the fermentation of sucrose by the bacterium, Leuconostoc mesenteroides (strain B-512). The crude material is composed of linked glucose units. In the fraction represented by dextran 40, 80% of the molecules have ...